Human IgM mu Antibody
ELISA of Goat Anti-Human IgM antibody
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Datasheet

Human IgM mu Antibody

Goat Polyclonal

609-1107
1 mg
ELISA, SDS-PAGE, Purification
Human
Goat
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Description

Background

Anti-Human IgM (mu heavy chain) generated in goat detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

Application Note

Anti-Human IgM mu heavy chain has been tested by ELISA and SDS-PAGE and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Purity/Specificity

Anti-Human IgM mu heavy chain was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum.   No reaction was observed against other human heavy or light chain proteins.

Anti-HUMAN IgM (mu chain) (GOAT) Antibody - 609-1107
goat anti-Human IgM mu chain Antibody, Gt-a-Human IgM, Human IgM Antibody in Goat, Human Secondary Antibody
ELISA, SDS-PAGE
Purification
Human
Human
Goat
Polyclonal
IgG
Native Protein
Human IgM whole molecule
Liquid (sterile filtered)
1.1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Wet Ice
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Expiration date is one (1) year from date of opening.
Yang Q et al. Analysis of pre-existing IgG and IgM antibodies against polyethylene glycol (PEG) in the general population. Anal Chem. (2016)
Applications
E, EIA
Campbell CT, Llewellyn SR, Demberg T, Morgan IL, Robert-Guroff M, Gildersleeve JC. High-throughput profiling of anti-glycan humoral responses to SIV vaccination and challenge. PLoS One. (2013)
Applications
Ab Immobilization

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.