Golden Syrian & Armenian Hamster IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed
Dylight conjugated antibody - Immunofluorescence Microscopy
DyLight™ 488 Fluorescence Spectra.
Properties of DyLight™ Fluorescent Dyes.
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Datasheet

Golden Syrian & Armenian Hamster IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed

Goat Polyclonal

620-141-440
100 µg
WB
Goat
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Description

Background

Anti-Golden Syrian & Armenian Hamster IgG DyLight Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Anti-Golden Syrian & Armenian Hamster IgG DyLight Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian & Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Golden Syrian & Armenian Hamster IgG and Golden Syrian & Armenian Hamster Serum.  No reaction was observed against Mouse or Rat Serum Proteins.  This antibody will react with heavy chains of Golden Syrian & Armenian Hamster IgG and with light chains of most Golden Syrian & Armenian Hamster immunoglobulins.

Anti-GOLDEN SYRIAN & ARMENIAN HAMSTER IgG (H&L) (GOAT) Antibody DyLight™ 488 Conjugated (Min X MOUSE and RAT Serum Proteins) - 620-141-440
goat anti-Golden Syrian & Armenian Hamster IgG DyLight™488 conjugated antibody, goat anti-Hamster IgG DyLight™ 488 conjugated antibody
WB
Armenian and Golden Syrian Hamster
Goat
DyLight™ 488
Polyclonal
IgG
Armenian and Golden Syrian Hamster IgG, whole molecule
4.2 moles DL488 per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.