Golden Syrian & Armenian Hamster IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
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Datasheet

Golden Syrian & Armenian Hamster IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Goat Polyclonal

620-105-440
1 mg
ELISA
Goat
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Description

Background

Anti-Golden Syrian & Armenian Hamster IgG Alkaline Phosphatase Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Anti-Golden Syrian & Armenian Hamster IgG Alk Phos conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian/Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Armenian/Golden Syrian Hamster IgG and Armenian/Golden Syrian Hamster Serum.  No reaction was observed against Mouse or Rat Serum Proteins.

Anti-GOLDEN SYRIAN & ARMENIAN HAMSTER IgG (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated (Min X MOUSE and RAT Serum Proteins) - 620-105-440
goat anti-Golden Syrian & Armenian Hamster IgG alkaline phosphatase conjugated antibody, goat anti-Hamster IgG alk phos conjugated antibody
ELISA
Armenian and Golden Syrian Hamster
Goat
Alkaline Phosphatase (Calf Intestine)
Polyclonal
IgG
Armenian and Golden Syrian Hamster IgG, whole molecule
Liquid (sterile filtered)
1 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.09% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.