Goat IgG (H&L) Antibody ATTO 647N Conjugated Pre-Adsorbed
Atto Dye-Immunoflourescence microscopy
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Goat IgG (H&L) Antibody ATTO 647N Conjugated Pre-Adsorbed

Rabbit Polyclonal

605-456-013S 605-456-013
100 µg 500 µg
Dot Blot, WB
Rabbit
Have a Question? Ask a Specialist
$99.00 /Per Item
$318.00 /Per Item
100 µg $99.00
500 µg $318.00
Availability: Ships next business day Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Note

Anti-Goat IgG (H&L) conjugated by ATTO 647N has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specificity

Goat IgG (H&L) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.  No reaction was observed against Human, Mouse or Rabbit Serum Proteins.  This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.

Storage Condition

Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Anti-GOAT IgG (H&L) (RABBIT) Antibody ATTO 647N Conjugated (Min X Hu, Ms, Rb Serum Proteins) - 605-456-013
rabbit anti-Goat IgG ATTO 647N Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO647N Conjugation
Dot Blot, WB
Goat
Rabbit
ATTO 647N
Polyclonal
IgG
2.5 moles AT647N per mole of IgG
Lyophilized
Ambient
Expiration date is one (1) year from date of opening.
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Restore with deionized water (or equivalent)
500 µL

Invalid lot number

If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.