Goat IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed
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Datasheet

Goat IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Donkey Polyclonal

605-706-125
1 mg
ELISA, IHC, WB
Goat
Donkey
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Description

Background

Anti-Goat IgG Biotin Antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Anti-Goat IgG (H&L) Antibody is ideal for researchers in Immunology, Cancer, and Microbiology research.

Application Note

Goat IgG Biotin Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Purity/Specificity

Anti-Goat IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Goat IgG and Goat Serum.  No reaction was observed against Chicken, Guinea Pig, Horse, Hamster, Mouse, Rabbit and Rat Serum Proteins.

Anti-GOAT IgG (H&L) (DONKEY) Antibody Biotin Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) - 605-706-125
donkey anti-Goat IgG Antibody biotin Conjugation, donkey anti-Goat IgG biotin Conjugated Antibody
ELISA
IHC, WB
Goat
Donkey
IgG (H&L)
Biotin
Polyclonal
IgG
Native Protein
Anti-Goat IgG (H&L) was produced by repeated immunization with goat IgG whole molecule in donkey.
10-20 moles BAC per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
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Applications
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Ambrosetti V et al. Characterizing the impact of maternal obesity on offspring ovarian development in rats. Endocrine. (2016)
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IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.