F(ab')2 Rabbit IgG [H&L] Antibody Phycoerythrin conjugated Pre-adsorbed
WB - F(ab')2 RABBIT IgG [H&L] Antibody Phycoerythrin conjugated Pre-adsorbed
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Datasheet

F(ab')2 Rabbit IgG [H&L] Antibody Phycoerythrin conjugated Pre-adsorbed

Donkey Polyclonal

711-708-127
500 µg
WB
Rabbit
Donkey
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Description

Background

F(ab')2 Anti-Rabbit IgG [H&L] Antibody Phycoerythrin Conjugated Pre-adsorbed was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Rabbit IgG [H&L] Antibody Phycoerythrin conjugated Pre-adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Application Note

F(ab')2 Rabbit IgG Phycoerythrin Conjugated Antibody was tested in WB, and is suitable for immunomicroscopy, flow cytometry or FACS analysis, as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Purity/Specificity

F(ab')2 Anti-Rabbit IgG (H&L) Phycoerythrin Conjugated Antibody Pre-adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.

F(ab')2 Anti-RABBIT IgG [H&L] (DONKEY) Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins - 711-708-127
Donkey F(ab')2 Anti-Rabbit IgG Phycoerythrin Conjugated Antibody, Donkey Fab2 Anti-Rabbit IgG Antibody PE Conjugation
WB
Rabbit
Rabbit
Donkey
R-Phycoerythrin (RPE)
Polyclonal
IgG F(ab')2
Native Protein
Fab2 Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole molecule in donkey.
Lyophilized
0.5 mg/mL by absorbance = 82.0 at 565 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.