F(ab')2 Bovine IgG (H&L) Antibody Texas Red™ Conjugated
Fab2 Bovine IgG Antibody Texas Red Conjugated - Dot Blot
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Datasheet

F(ab')2 Bovine IgG (H&L) Antibody Texas Red™ Conjugated

Rabbit Polyclonal

701-409-002
500 µL
Dot Blot
Bovine
Rabbit
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$147.00 /Per Item
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Description

Background

F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Application Note

F(ab')2 Anti-Bovine IgG Texas Red Conjugated Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Bovine IgG and Bovine Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

F(ab')2 Anti-BOVINE IgG [H&L] (RABBIT) Antibody Texas Red™ Conjugated - 701-409-002
Rabbit F(ab')2 Anti-Bovine Texas Red™ Conjugated Antibody, Rabbit Fab2 Anti-Bovine Texas Red™ Conjugated Antibody, Rabbit Fab'2 Anti-Bovine Antibody Texas Red™ Conjugation
Dot Blot
Bovine
Bovine
Rabbit
Texas Red®
Polyclonal
IgG F(ab')2
Bovine IgG whole molecule
1.6 moles TR per mole of IgG F(ab')2
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
500 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.