F(ab')2 Bovine IgG (H&L) Antibody Phycoerythrin conjugated

Datasheet

F(ab')2 Bovine IgG (H&L) Antibody Phycoerythrin conjugated

Name: F(ab')2 Bovine IgG (H&L) Antibody Phycoerythrin conjugated
Brand: Rockland Immunochemicals
Price: 275.0 USD
Availability: InStock

Rabbit Polyclonal

Item No. 701-408-002

Size: 500 µg

Reactivity: Bovine

Host Species: Rabbit

$275.00 /Per Item

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Background

F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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Specifications for F(ab')2 Bovine IgG (H&L) Antibody Phycoerythrin conjugated

Product Details

Description: Rabbit F(ab')2 Anti-Bovine IgG (H&L) Antibody Phycoerythrin Conjugated - 701-408-002

Synonyms: Rabbit F(ab')2 Anti-Bovine Phycoerythrin Conjugated Antibody, Rabbit Fab2 Anti-Bovine Phycoerythrin Conjugated Antibody, Rabbit Fab'2 Anti-Bovine Antibody PE Conjugation

Host Species: Rabbit

Specificity: IgG (H&L)

Conjugate: R-Phycoerythrin (RPE)

Clonality: Polyclonal

Format: IgG F(ab')2

Target Details

Reactivity: Bovine

Immunogen: Bovine IgG whole molecule

Purity/Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rabbit Serum, Bovine IgG and Bovine Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Application Details

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Formulation

Physical State: Lyophilized

Concentration: 1.0 mg/mL by absorbance = 82.0 at 565 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000)

Reconstitution Volume: 500 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Expiration: Expiration date is one (1) year from date of receipt.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.