Fab Sheep IgG (H&L) Antibody Peroxidase Conjugated
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Datasheet

Fab Sheep IgG (H&L) Antibody Peroxidase Conjugated

Donkey Polyclonal

813-7302
1 mg
Sheep
Donkey
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$218.00 /Per Item
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Description

Background

Fab Anti-Sheep IgG (H&L) Antibody generated in donkey detects immunoglobulin g from sheep, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Fab Anti-Sheep IgG Antibody is conjugated to Peroxidase.

Application Note

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum.  No reaction was observed against anti-Papain or anti-Donkey IgG F(c).

Fab Anti-SHEEP IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated - 813-7302
Donkey Fab Anti-Sheep IgG Antibody Peroxidase Conjugation, Donkey Fab Anti-Sheep IgG HRP Conjugated Antibody
Sheep
Donkey
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG Fab
Sheep IgG whole molecule
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.