Chicken IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
WB - Chicken IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
Western Blot of Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins)
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Datasheet

Chicken IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Goat Polyclonal

603-105-126
1 mg
Dot Blot, ELISA, WB
Chicken
Goat
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Description

Background

Anti-Chicken IgG Alkaline Phosphatase Antibody generated in goat detects chicken IgY. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Note

Anti-Chicken IgG Alkaline Phosphatase Conjugate has been tested by ELISA, dot blot, and western blot. This product is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Chicken IgG and Chicken Serum.   No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

Anti-CHICKEN IgG (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) - 603-105-126
Alk Phos Chicken Antibody, Gt-a-Chicken IgG Alkaline Phosphatase, Chicken Antibody in Goat, Chicken Secondary Antibody, Alk Phos Gt anti-Ch IgY Antibody, Goat Anti-Chicken IgY Alkaline Phosphatase,
Dot Blot, ELISA, WB
Chicken
Chicken
Goat
Alkaline Phosphatase (Calf Intestine)
Polyclonal
IgG
Native Protein
Anti-Chicken IgG whole molecule was produced by repeated immunization with Chicken IgG whole molecule in goat.
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.