Swine IgG F(ab')2 Peroxidase
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Datasheet

Swine IgG F(ab')2 Peroxidase

014-0304
1 mg
Swine
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Description

Background

Swine IgG F(ab')2 Fragment Peroxidase Conjugated is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Swine IgG F(ab')2 molecules lack the Fc portion of Swine IgG and therefore receptors that bind Swine IgG Fc will not bind Swine IgG F(ab')2 molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region followed by peroxidase conjugation.

Application Note

Swine IgG F(ab')2 fragment reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Purity/Specificity

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Swine IgG, anti-Swine IgG F(ab’)2 and anti-Swine Serum.  No reaction was observed against anti-Swine IgG F(c) or anti-Pepsin.

SWINE IgG F(ab')2 fragment Peroxidase conjugated - 014-0304
Swine IgG F(ab')2 fragment peroxidase conjugation, Swine IgG F(ab')2 fragment HRP conjugate
Swine
Peroxidase (Horseradish)
IgG F(ab')2
Protein
Native Protein
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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