Datasheet

Swine IgG F(ab')2

Name: Swine IgG F(ab')2
Brand: Rockland Immunochemicals
Availability: InStock

Item No. 014-0104

Size: 2 mg

Applications: SDS-PAGE

Origin: Swine

Please contact us for pricing

2 mg $0.00

1 mg $210.00

Add to List

Bulk Orders

Availability: Ships next business day

Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Swine IgG F(ab')2 Fragment is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Swine IgG F(ab')2 molecules lack the Fc portion of Swine IgG and therefore receptors that bind Swine IgG Fc will not bind Swine IgG F(ab')2 molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Expand Background

Specifications for Swine IgG F(ab')2

Product Details

Description: Swine IgG F(ab')2 Fragment (BULK ORDER) - 014-0104

Synonyms: Swine IgG F(ab')2 fragment, Swine IgG Fab2 fragment

Species of Origin: Swine

Format: IgG F(ab')2

Type: Native Protein

Target Details

Purity/Specificity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine IgG, anti-Swine IgG F(ab’)2 and anti-Swine Serum. No reaction was observed against anti-Swine IgG F(c) or anti-Pepsin.

Application Details

Tested Applications: SDS-PAGE

Application Note: Swine IgG F(ab')2 Fragment has been tested by SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 2.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiration: Expiration date is one (1) year from date of receipt.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.