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Datasheet

Swine IgG F(ab')2

014-0104 014-0104-0001
2 mg 1 mg
Liquid (sterile filtered) Liquid (sterile filtered)
SDS-PAGE
Swine
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$210.00 /Per Item
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Background

Swine IgG F(ab')2 Fragment is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Swine IgG F(ab')2 molecules lack the Fc portion of Swine IgG and therefore receptors that bind Swine IgG Fc will not bind Swine IgG F(ab')2 molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Product Details

Swine IgG F(ab')2 Fragment (BULK ORDER) - 014-0104
Swine IgG F(ab')2 fragment, Swine IgG Fab2 fragment
Swine
IgG F(ab')2
Native Protein

Target Details

This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine IgG, anti-Swine IgG F(ab’)2 and anti-Swine Serum.  No reaction was observed against anti-Swine IgG F(c) or anti-Pepsin.

Application Details

SDS-PAGE
Swine IgG F(ab')2 Fragment has been tested by SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Formulation

2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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