Rabbit IgG Biotin conjugated
SDS-PAGE of Rabbit IgG Whole Molecule Biotin Conjugated (p/n 011-0602)
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Rabbit IgG Biotin conjugated

011-0602
1 mg
FC
Have a Question? Ask a Specialist
$108.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-afinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This Human IgG whole molecule is conjugated to biotin (Vitamin H), a small biomolecule that has a large affinity for avidin and streptavidin. Rabbit IgG whole molecule Biotin Conjugated is ideal for investigators in Immunology, Cancer, and Microbiology research.

Application Note

Rabbit IgG whole molecule Biotin conjugated can be utilized as a control reagent in both Western Blotting and ELISA format experiments. A streptavidin-conjugated reporter (ie. Streptavidin Peroxidase Conjugated) can be used for detection.

Purity/Specificity

Rabbit IgG whole molecule Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Rabbit IgG whole molecule Biotin conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit IgG and anti-Rabbit Serum.

RABBIT IgG whole molecule Biotin conjugated - 011-0602
Rabbit IgG Biotin conjugation, Biotin conjugated Rabbit IgG whole molecule
FC
Rabbit
Biotin
IgG
Protein
Native Protein
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Rabbit IgG whole molecule Biotin conjugated is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Heukels et al. Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis. Respiratory Research (2018)
Applications
FC, FACS, FLOW

Invalid lot number

If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related products to: Rabbit IgG Biotin conjugated