Datasheet

Rabbit IgG

Name: Rabbit IgG
Brand: Rockland Immunochemicals
Availability: InStock

13 References

Item No. 011-0102

Size: 50 mg

Applications: IF, FC, SDS-PAGE, IP, LFA

Origin: Rabbit

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50 mg $0.00

1 g $0.00

10 mg $157.00

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Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

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Specifications for Rabbit IgG

Product Details

Description: Rabbit IgG Whole Molecule (BULK ORDER) - 011-0102

Synonyms: Rabbit immunoglobulin G

Species of Origin: Rabbit

Format: IgG

Type: Native Protein

Target Details

Purity/Specificity: Rabbit IgG whole molecular was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Rabbit IgG whole molecular was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.

Application Details

Tested Applications: SDS-PAGE

Suggested Applications: FC, IF, IP, LFA - View References

Application Note: Rabbit IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation

Physical State: Lyophilized

Concentration: 10.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Reconstitution Volume: 5.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Rabbit IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (13)

Chen Z, Zhang Z, Zhai X, et al. (2020). Rapid and Sensitive Detection of anti-SARS-CoV-2 IgG, Using Lanthanide-Doped Nanoparticles-Based Lateral Flow Immunoassay. Anal Chem.

Applications

Lateral Flow (LFA)

PubMed

Stoltenburg R et al. (2018). Refining the Results of a Classical SELEX Experiment by Expanding the Sequence Data Set of an Aptamer Pool Selected for Protein A. Int J Mol Sci.

Applications

SPR-Based Analyses

PubMed

Heras S et al. (2017). Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during pronuclear development in equine zygotes produced by ICSI. Epigenetics Chromatin.

Applications

IF, Confocal Microscopy

PubMed

Liang RL. et al. (2017). Europium (III) chelate microparticle-based lateral flow immunoassay strips for rapid and quantitative detection of antibody to hepatitis B core antigen. Sci Rep.

Applications

Lateral Flow (LFA)

PubMed

Heras S et al. (2016). Determination of the parental pronuclear origin in bovine zygotes: H3K9me3 versus H3K27me2-3. Anal Biochem.

Applications

Undefined

PubMed

Mounir, Z et al. (2016). ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. ELife

Applications

IP, Co-IP

PubMed

Wang, Q et al. (2016). Altered Expression of IFN-λ2 in Allergic Airway Disorders and Identification of Its Cell Origins. Mediators of Inflammation

Applications

FC, FACS, FLOW

PubMed

Heras S et al. (2015). Asymmetric histone 3 methylation pattern between paternal and maternal pronuclei in equine zygotes. Anal Biochem.

Applications

Control

PubMed

Heras S et al. (2014). DNA counterstaining for methylation and hydroxymethylation immunostaining in bovine zygotes. Anal Biochem.

Applications

Control

PubMed

Ying G et al. (2014). Centrin 2 is required for mouse olfactory ciliary trafficking and development of ependymal cilia planar polarity. J Neurosci.

Applications

IP, Co-IP

PubMed

Cao D et al. (2009). Combined proteomic–RNAi screen for host factors involved in human hepatitis delta virus replication. RNA.

Applications

IP, Co-IP

PubMed

Moore RW et al. (2003). Effect of bursal anti-steroidogenic peptide and immunoglobulin G on neonatal chicken B-lymphocyte proliferation. Comp Biochem Physiol C Toxicol Pharmacol.

Applications

Other

PubMed

White CA et al. (1998). Microglia are more susceptible than macrophages to apoptosis in the central nervous system in experimental autoimmune encephalomyelitis through a mechanism not involving Fas (CD95). Int Immunol.

Applications

FC, FACS, FLOW

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Biotin Binding Assay Protocol

Flow Cytometry (FC) Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Immunoprecipitation (IP) Protocol

IP-WB with TrueBlot® Protocol

Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.