Horse IgG F(c) fragment
WB - Horse IgG F(c) fragment
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Horse IgG F(c) fragment

008-0103
1 mg
SDS-PAGE, IHC
Horse
Have a Question? Ask a Specialist
$169.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.

Application Note

Horse IgG F(c) Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Purity/Specificity

Horse IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Horse IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(c).  No reaction was observed against anti-Horse IgG F(ab’)2 or anti-Papain.

HORSE IgG F(c) fragment - 008-0103
Horse immunoglobulin G F(c), IgG F(c), Horse Fc Fragment
SDS-PAGE
IHC
Horse
IgG F(c)
Protein
Native Protein
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
1.0 mL
Restore with deionized water (or equivalent)
Wet Ice
Store vial at 4° C prior to restoration.   Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Horse IgG F(c) fragment is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Herb VM et al. Immunohistochemical staining of immunoglobulin G in healthy equine, canine, and feline corneas. Vet Opthalmol. (2022)
Applications
IHC, ICC, Histology

Invalid lot number

If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related products to: Horse IgG F(c) fragment