Green Fluorescent Protein (GFP) Control Protein
Anti-GFP Chemiluminescent Kit for Western blot
SDS-PAGE results of recombinant GFP protein
Immunoprecipitation and Western Blot using GFP Protein
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Datasheet

Green Fluorescent Protein (GFP) Control Protein

000-001-215
100 µg
SDS-PAGE, WB, Microarray
Aequorea victoria
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Description

Background

Green Fluorescent Protein (GFP) is used as a positive control when detecting GFP fusion proteins in Anti-GFP antibody assays. Rockland offers multiple purified hosts and conjugated Anti-GFP that can detect GFP by ELISA (sandwich or capture) or western blot. Biotin conjugated polyclonal Anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution when used in combination with monoclonal Anti-GFP (p/n 600-301-215), using either form of the antibody as the capture or detection antibody.

Application Note

GFP Control is suitable as a control for polyclonal or monoclonal Anti-GFP in immunological assays. Researchers should determine optimal titers for applications. The molecular weight of GFP is 28 kDa. Green Fluorescent Protein has been tested in SDS-page, western blot, and immunoprecipitation.

Purity/Specificity

GFP control protein was expressed in E. coli and purified by sequential hydrophobic interaction chromatography, DEAE chromatography and size-exclusion chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-GFP. Analysis by SDS-PAGE resulted in a pattern consistent with purified GFP and was estimated to be greater than 90% pure.

Recombinant Green Fluorescent Protein (GFP) Control Protein - 000-001-215
control protein, rGFP protein, GFP fusion protein, GFP control
SDS-PAGE, WB
Microarray
 - View References
Aequorea victoria
E. coli
Liquid (sterile filtered)
1mg/ml by BCA assay
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store GFP at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is six (6) months from date of receipt.
Direct Correlation between Motile Behavior and Protein Abundance in Single Cells. PLOS Computational Biology ()
Applications
WB, IB, PCA
Direct correlation between motile behavior and protein abundance in single cells. PLoS Comput Biol. ()
Applications
WB, IB, PCA
Antibody colocalization microarray: a scalable technology for multiplex protein analysis in complex samples. Molecular & Cellular Proteomics : Mcp ()
Applications
Microarray, Microarray chip

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.