VDAC/Porin Antibody
Anti-VDAC/Porin Antibody - Western Blot
Rabbit anti-VDAC/Porin 1 WB
Rabbit anti-VDAC-Porin WB
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Datasheet

VDAC/Porin Antibody

Rabbit Polyclonal

600-401-882S 600-401-882
25 µL 200 µg
ELISA, WB
Human, Mouse, Rat
Rabbit
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$99.00 /Per Item
$333.00 /Per Item
25 µL $99.00
200 µg $333.00
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Description

Background

VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger - see below).  The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mito-chondrial membrane.  VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis.  VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.  This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations.

Application Note

VDAC/Porin Antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract.

Purity/Specificity

Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms).  Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).

Anti-VDAC/Porin (RABBIT) Antibody - 600-401-882
rabbit anti-VDAC/Porin antibody, VDAC-1 Loading Control Antibody, Porin Loading Control Antibody, VDAC-1 Antibody, VDAC1 Antibody, POR1 Antibody, Porin Antibody, Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1
VDAC1
ELISA, WB
Human
Human, Mouse, Rat
Rabbit
Polyclonal
IgG
Peptide
VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1.
Liquid (sterile filtered)
1.0mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store VDAC antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Wang PY et al. Reducing fatty acid oxidation improves cancer-free survival in a mouse model of Li-Fraumeni syndrome. Cancer Prev Res (Phila). (2021)
Applications
WB, IB, PCA
Maddalena LA et al. The mitochondria-targeted imidazole substituted oleic acid 'TPP-IOA'affects mitochondrial bioenergetics and its protective efficacy in cells is influenced by cellular dependence on aerobic metabolism. Biochim Biophys Acta Bioenerg. (2017)
Applications
WB, IB, PCA
Thakurela et al. The transcriptome of mouse central nervous system myelin. Scientific Reports (2016)
Applications
WB, IB, PCA
Petermann P et al. Impact of Rac1 and Cdc42 signaling during early herpes simplex virus type 1 infection of keratinocytes. J Virol. (2009)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.