TrkCT1 Antibody
Anti-TrkCT1 Antibody - Western Blot
Anti-TrkCT1 Antibody - Immunoprecipitation/Western Blot
Anti-TrkCT1 Antibody - Western Blot
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Datasheet

TrkCT1 Antibody

Rabbit Polyclonal

600-401-993
100 µg
ELISA, IP, WB
Human, Mouse
Rabbit
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$399.00 /Per Item
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. TrkCT1, also named neurotrophic tyrosine kinase receptor type 3 (Ntrk3) non-catalytic isoform 2, is a non-catalytic isoform of TrkC, the high affinity receptor for Neurotrophin-3 (NT-3).  This isoform lacks the kinase domain that is responsible for signaling by the full-length isoform.  TrkCT1 is the product of an alternative splicing of Ntrk3 that leaves the extracellular and transmembrane domains intact but includes a shorter intracellular domain encoded by exons 13b and 14b.  Recent studies indicate that the short cytoplasmic tail binds the scaffold protein Tamalin in a ligand-dependent manner and further activates the Arf6–Rac1 signaling pathway. Isoform 3 transcripts are readily detected early during embryogenesis and are expressed predominantly in adult brain and gonads.

Application Note

This affinity purified antibody has been tested for use in ELISA, IP, and western blotting. Specific conditions for reactivity should be optimized by the end user.  While the predicted molecular weight for TrkCT1 is approximately 68 kDa, the protein has been reported to migrate at about 95 kDa on western blots.  The molecular weight noted for TrkCT1 depends somewhat upon the cell lysate or extract used for western blotting, and this variation may be due to post-translational modifications of the protein.

Purity/Specificity

This affinity purified antibody is directed against mouse TrkCT1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross-reactivity with TrkCT1 protein from human, mouse and rat based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.

Anti-TrkCT1 (RABBIT) Antibody - 600-401-993
rabbit anti-TrkCT1 antibody, rabbit anti-Ntrk3 antibody, TrkCT-1, NT-3 growth factor receptor, GP145-TrkC, Trk-C, ETV6 NTRK3 fusion antibody, GP145 TrkC antibody, GP145TrkC antibody, Neurotrophic tyrosine kinase receptor type 3 antibody, TrkC tyrosine kinase, TrkC NC2
Ntrk3
ELISA, IP, WB
Mouse
Human, Mouse
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse TrkCT1 protein.
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Dedoni S et al. The neurotrophin receptor trkc as a novel molecular target of the antineuroblastoma action of valproic acid. Int J Mol Sci. (2021)
Applications
IP, Co-IP; WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.