TAF1 Antibody
Immunohistochemistry of rabbit anti-TAF1 antibody
Anti-TAF1 Antibody - Western Blot
Anti-TAF1 Antibody - Western Blot
CHIP - Anti- TAF1 Antibody
Rabbit anti-TAF1
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Datasheet

TAF1 Antibody

Rabbit Polyclonal

600-401-995
100 µg
ChIP, ELISA, WB
Human, Mouse
Rabbit
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$399.00 /Per Item
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Description

Background

Anti-TAF1 antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors.  TFIID nucleates the formation of transcription pre-initiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II.  TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53.  TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1).  The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity.  TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18% of mammalian genes.

Application Note

This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, ChIP, and western blotting. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 213 kDa in size corresponding to TAF1 protein by western blotting in the appropriate cell lysate or extract.

Purity/Specificity

This affinity purified antibody is directed against human TAF1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross-reactivity with TAF1 protein from human, mouse and rat based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.

Anti-Transcription Initiation Factor TFIID Subunit 1 (TAF1) (RABBIT) Antibody - 600-401-995
Rabbit anti-TAF1 antibody, rabbit anti-Anti-Transcription Initiation Factor TFIID Subunit 1 antibody, TAF-1, TAF 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, TAFII-250
TAF1
ChIP, ELISA, WB
Human
Human, Mouse
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human TAF1.
Liquid (sterile filtered)
1.1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Gegonne A et al. The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells. Mol Cell Biol. (2012)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.