Stat5 phospho Y694 Antibody
Anti-Stat5 pY694 Antibody - Immunohistochemistry
Anti-Stat5 pY694 Antibody - Western Blot
Anti-Stat5 pY694 Antibody - Immunofluorescence Microscopy
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Datasheet

Stat5 phospho Y694 Antibody

Mouse Monoclonal 5F6.F1 IgG1 kappa

200-301-B18
1 mg
ELISA, IF, IHC, WB
Human, Mouse
Mouse
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Description

Background

Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5 alpha and Stat5 beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.

Application Note

This monoclonal antibody has been tested by ELISA, immunofluorescence, immunohistochemistry, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific monoclonal antibody reacts with mouse Stat5a phospho Y694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Purity/Specificity

Anti-STAT5 phospho Y694 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for mouse Stat5a protein phosphorylated at Y694.  A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence.  Cross-reactivity with Stat5a from other sources has not been determined.

Storage Condition

Store Anti-STAT5 phospho Y694 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Phospho STAT5 pY694 Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-STAT5 pY694 (MOUSE) Monoclonal Antibody - 200-301-B18
mouse anti-STAT5 pY694 Antibody, MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5 antibody
Stat5a
ELISA, IF, IHC, WB
Mouse
Human, Mouse
Phosphorylation
Mouse
Monoclonal
5F6.F1
IgG1
Peptide
Phospho STAT5 pY694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
Liquid (sterile filtered)
Dry Ice
Expiration date is one (1) year from date of opening.
1mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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