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Datasheet

Six3 Antibody

Rabbit Polyclonal

6 References
600-401-A26S 600-401-A26
25 µL 100 µg
Liquid (sterile filtered) Liquid (sterile filtered)
WB, ELISA, IHC, IF, Multiplex
Mouse
Rabbit
$125.00 /Per Item
$429.00 /Per Item
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Background

Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke’s pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Product Details

Anti-Homeobox protein SIX3 (RABBIT) Antibody - 600-401-A26
rabbit anti-Six3 antibody, Six-3, Six 3, Homeobox protein SIX3, Sine oculis homeobox homolog 3
Rabbit
Polyclonal
IgG

Target Details

Six3  - View All Six3 Products
Mouse
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody reacts with mouse Six3.   Cross-reactivity with Six3 from other sources has not been determined.
Q62233 - UniProtKB
59939908 - NCBI Protein

Application Details

ELISA, IHC, WB
IF, Multiplex  - View References
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

Formulation

1.09 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development. Neuron
Applications
Undefined
(). Generation and Characterization of Induced Pluripotent Stem Cells and Retinal Organoids From a Leber's Congenital Amaurosis Patient With Novel RPE65 Mutations. Frontiers in Molecular Neuroscience
Applications
IF, Confocal Microscopy
(). PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice. ELife
Applications
IHC, ICC, Histology
(). SP8 and SP9 coordinately promote D2-type medium spiny neuron production by activating Six3 expression. Development
Applications
IHC, ICC, Histology; Multiplex Assay
(). Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells. Stem Cells International
Applications
IF, Confocal Microscopy; WB, IB, PCA
(). Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation. Cell Reports
Applications
IHC, ICC, Histology; Multiplex Assay

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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