Selenoprotein W Antibody
Anti-SeIW Antibody - Western Blot
Proposed pathway for Selenoprotein W function.
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Datasheet

Selenoprotein W Antibody

Rabbit Polyclonal

600-401-A29S 600-401-A29
25 µL 100 µg
ELISA, WB, IHC
Mouse
Rabbit
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$99.00 /Per Item
$399.00 /Per Item
25 µL $99.00
100 µg $399.00
Availability: Ships next business day Availability: Ships on approximately 01/13/2023
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Selenoprotein W was first purified and characterized from rat skeletal muscle.  The function of selenoprotein W is not entirely clear, but the presence of the bound glutathione moiety indicates that SelW is thought to function in oxidation–reduction catalysis and may play a role in selenium deficiency disorders such as white muscle disease in sheep and Keshan disease in humans. Recently, overexpression of SelW was shown to be glutathione dependent and was shown to markedly reduce the sensitivity of cell lines to H2O2 cytotoxicity.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 9.6 kDa in size corresponding to SelW by western blotting in the appropriate cell lysate or extract.  This antibody is capable of detecting both overexpressed and endogenous SelW.

Purity/Specificity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for mouse Selenoprotein W.  A BLAST analysis was used to suggest cross-reactivity with SelW from rat based on a 98% homology with the immunizing sequence.  Partial reactivity is expected against human-derived SelW based on an 87% homology with the immunogen.  Cross-reactivity with SelW from other sources has not been determined.

Anti-Selenoprotein W (RABBIT) Antibody - 600-401-A29
rabbit anti-Selenoprotein W antibody, Rabbit anti-SelW antibody, Selenow, Sepw1
Selenow
ELISA, WB
IHC
Mouse
Mouse
Rabbit
Polyclonal
IgG
Recombinant Protein
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full-length mouse SelW protein.
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Kang D et al. Selenophosphate synthetase 1 deficiency exacerbates osteoarthritis by dysregulating redox homeostasis. Nat Commun. (2022)
Applications
IHC, ICC, Histology; WB, IB, PCA
Schwarz M et al. Copper interferes with selenoprotein synthesis and activity. Redox Biol. (2020)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.