SARS-CoV-2 Nucleocapsid (N) Protein Antibody
Western Blot Results of Rabbit Anti-SARS CoV-2 Nucleocapsid (N) Protein Antibody
ELISA results of Rabbit Anti-SARS CoV-2 Nucleocapsid (N) Protein.
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Datasheet

SARS-CoV-2 Nucleocapsid (N) Protein Antibody

Rabbit Polyclonal

200-401-MS4-0100 200-401-MS4
100 µg 500 µg
ELISA, WB
SARS-CoV-2
Rabbit
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$253.00 /Per Item
$456.00 /Per Item
100 µg $253.00
500 µg $456.00
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Description

Background

SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2 or COVID-19) is related to SARS-CoV, MERS, and four milder coronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive-strand RNA virus that consists of four structural proteins: spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein. The spike protein is the most important surface protein of coronavirus. SARS-CoV-2 has a high affinity binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial. ACE2 is a membrane-bound aminopeptidase that has a vital role in the cardiovascular and immune systems. The nucleocapsid protein is a most abundant protein of coronavirus. The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody is useful for researchers interested in diagnostics and viral research.

Application Note

Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody has been tested for use in ELISA and Western Blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract.

Purity/Specificity

This protein A purified antibody is directed against SARS Coronavirus 2 Nucleocapsid (N) protein. The product was purified from monospecific antiserum by protein A affinity purification.  BLAST analysis was used to suggest reactivity with related Coronavirus proteins.  Cross reactivity with homologues from other sources has not been determined.

Anti-SARS-CoV-2 Nucleocapsid (N) Protein (RABBIT) Antibody - 200-401-MS4
rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, N-protein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2
N
ELISA, WB
SARS-CoV-2
Rabbit
Polyclonal
IgG
Recombinant Protein
Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
Lyophilized
5.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
500 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Hasanpourghadi, M et al. T cell responses to adenoviral vectors expressing the SARS-CoV-2 nucleoprotein. Current Trends in Microbiology (2021)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.