Datasheet

OspC Antibody

Name: OspC Antibody
Brand: Rockland Immunochemicals
Price: 125.0 USD
Availability: InStock

Rabbit Polyclonal

3 References

Item No. 200-401-C11

Size: 100 µg

Applications: WB, ELISA, IF

Reactivity: Borrelia burgdorferi

Host Species: Rabbit

$475.00 /Per Item

25 µL $125.00

100 µg $475.00

Add To Cart

Add to List

Bulk Orders

Availability: Ships next business day

Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane.

Expand Background

Specifications for OspC Antibody

Product Details

Description: Anti-OspC (RABBIT) Antibody - 200-401-C11

Synonyms: rabbit anti-OspC Antibody, Borrelia burgdorferi OspC, PC, Outer Surface Protein C, BB_B19

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: ospC - View All ospC Products

Reactivity: Borrelia burgdorferi

Immunogen Type: Recombinant Protein

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.

Purity/Specificity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspC protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology. Cross-reactivity with OspC from other sources has not been determined.

Database Links:

UniProtKB - Q07337

NCBI - NP_047005.1

GeneID - 1194415

Application Details

Tested Applications: ELISA, WB

Suggested Applications: IF - View References

Application Note: This protein-A purified antibody has been tested for use in Western blotting and in ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.

Formulation

Physical State: Lyophilized

Concentration: 1.295 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (3)

Salverda, Merijn Louis Marten (Amsterdam, NL), Van Der, Ley Peter André (Utrecht, NL) (2020). Surface display of antigens on Gram-negative outer membrane vesicles. patent:20200197308

Applications

IF, Confocal Microscopy

Source

Caine et al. (2017). Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival. Cellular Microbiology

Applications

WB, IB, PCA

PubMed

Caine et al. (2015). A short-term Borrelia burgdorferi infection model identifies tissue tropisms and bloodstream survival conferred by adhesion proteins. Infection and Immunity

Applications

WB, IB, PCA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.