NOTCH 2 Antibody
Anti-NOTCH 2 Antibody - Immunohistochemistry
Rabbit anti-NOTCH2 WB Multilysate
Western Blot of Rabbit Anti-Notch 2 N-Term Antibody
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Datasheet

NOTCH 2 Antibody

Rabbit Polyclonal

100-401-408S 100-401-408
25 µL 200 µL
ELISA, IHC, WB
Human
Rabbit
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$99.00 /Per Item
$417.00 /Per Item
25 µL $99.00
200 µL $417.00
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Description

Background

Anti-Notch 2 Antibody recognizes Notch2 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Anti-NOTCH2 Antibody is useful for researchers interested in Notch1, Jagged1, Jagged2, and Delta1, as well as neuroscience, transcription and cancer research.

Application Note

Anti-Notch2 has been tested in ELISA, WB, and IHC. Anti-NOTCH-2 has a strong response was detected by ELISA against the immunizing peptide. This product was assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG [H&L] (Goat) (code #611-1302) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (code # ABTS-100) as a substrate for 30 minutes at room temperature.  A working dilution of 1:30,000 to 1:90,000 is suggested in ELISA this product.

Purity/Specificity

This antiserum is directed against human NOTCH2.  The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. Based on sequences, we expect this antibody to react as well with rat and mouse NOTCH 2. Only one amino acid difference (conservative) occurs in mouse and rat.

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-NOTCH 2 (Cleaved N terminal) (Human specific) (RABBIT) Antibody - 100-401-408
rabbit anti-Notch2 antibody, AGS2 antibody, hN2 antibody, Notch homolog 2 antibody, neurogenic locus notch homolog protein 2
NOTCH2
ELISA, IHC, WB
Human
Human
Rabbit
Polyclonal
Antiserum
Peptide
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 2 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Liquid (sterile filtered)
Dry Ice
Expiration date is one (1) year from date of opening.
75 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Iervolino A et al. Potassium depletion induces cellular conversion in the outer medullary collecting duct altering Notch signaling pathway. (2020)
Applications
WB, IB, PCA
Guilmeau S et al. Intestinal deletion of Pofut1 in the mouse inactivates notch signaling and causes enterocolitis. (2008)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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