Myosin 1G Antibody
Anti-Myosin 1G Antibody - Western Blot
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Datasheet

Myosin 1G Antibody

Rabbit Polyclonal

600-401-959
100 µg
ELISA, WB
Human
Rabbit
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$399.00 /Per Item
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. In general, myosins are protein complexes consisting of one or more myosin heavy chains, associated light chains and other proteins.  Myosins function as molecular motors and use the energy of ATP hydrolysis to move actin filaments or to move vesicles or other cargo on fixed actin filaments.  Myosins have magnesium-ATPase activity and bind actin. Myosins can be divided into classes that are distinguished based on sequence features of the motor, or head domain, but also have distinct tail regions that are believed to bind specific cargoes. Unconventional myosins exist.  Myosin 1G is an unconventional myosin that is restricted to hematopoietic cells. Unconventional myosins are also critical for motility in amoeba and a mammalian paralog (Myo1C) is critical as a glucose transporter that recycles glucose in response to insulin.

Application Note

This affinity-purified antibody has been tested for use in ELISA, western blotting and possibly in immunoprecipitation as well.  Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 100 kDa in size corresponding to Myosin 1G protein is expected in the appropriate cell lysate or extract.

Purity/Specificity

This affinity-purified antibody is directed against human Myosin 1G protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross reactivity with Myosin 1G from human, mouse, chimpanzee and rat based on 100% homology with the immunizing sequence. Cross reactivity with Myosin 1G from other sources has not been determined.

Anti-Myosin 1G (RABBIT) Antibody - 600-401-959
rabbit anti-Myosin 1G antibody, Unconventional myosin-Ig, Minor histocompatibility antigen HA-2, mHag HA-2, MYO1G
MYO1G
ELISA, WB
Human
Human
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region of human Myosin 1G protein.
Liquid (sterile filtered)
1.2 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Olety B et al. Myosin 1G (Myo1G) is a haematopoietic specific myosin that localises to the plasma membrane and regulates cell elasticity. FEBS Lett. (2010)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.