MMP2 Antibody
Immunofluorescence of Rabbit Anti-MMP2 Antibody
Immunofluorescence of Anti-MMP2 Antibody
Immunohistochemistry of Rabbit Anti-MMP2 Antibody
Western Blot of Rabbit Anti-MMP2 Antibody
Western Blot of Anti-MMP2 Antibody
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

MMP2 Antibody

Rabbit Polyclonal

600-401-MJ9S 600-401-MJ9
25 µL 100 µg
ELISA, IF, IHC, WB
Human
Rabbit
Have a Question? Ask a Specialist
$125.00 /Per Item
$449.00 /Per Item
25 µL $125.00
100 µg $449.00
Availability: Ships next business day Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Matrix metalloproteases (matrix metalloproteinase, MMPs), also called matrixins, are zinc-dependent endopeptidases and the major proteases in ECM degradation. MMPs can degrade several extracellular molecules and several bioactive molecules. MMP2 is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellularly by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, metastasis, tissue repair, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, it can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. MMP2 also cleaves KISS at a Gly- -Leu bond. It appears to have a role in myocardial cell death pathways and contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro and is involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. MMP2 is a ligand for integrin/beta3 on the surface of blood vessels. Anti-MMP2 antibody is useful for researchers interested in osteolysis, nodulosis, arthropathy, Apoptosis Research, and Cytokines & Growth Factor Antibodies.

Application Note

Anti-MMP2 Antibody has been tested in ELISA, WB, IF, and IHC.  Expect a band at ~ 65, 74kDa in western blot using appropriate lysates.  Positive control used: Human placenta and Human lung lysates, as well as HEK293T MMP2 overexpressed lysate in WB; HepG2 and U87MG cells for IF; Human metastatic breast carcinoma in lymph nodes for IHC.

Purity/Specificity

This affinity purified antibody is directed against human MMP2.  This product was affinity purified from monospecific antiserum by immunoaffinity purification.    A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse, rat, bovine, and Oryctolagus cuniculus.

Anti-MMP2 (RABBIT) Antibody - 600-401-MJ9
Rabbit Anti-MMP2 Antibody, Rabbit Anti-Matrix Metallopeptidase 2 Antibody, Matrix Metalloproteinase-2, CLG4A, MMP-2, TBE-1, Matrix Metalloproteinase 2 Gelatinase A 72kDa Type IV Collagenase), Matrix Metallopeptidase 2 Gelatinase A 72kDa Type IV Collagenase), Matrix Metalloproteinase-II, 72 KDa Type IV Collagenase, Collagenase Type IV-A, Neutrophil Gelatinase, 72 KDa Gelatinase, Gelatinase A, EC 3.4.24.24, MMP-II, MONA, CLG4
MMP2
ELISA, IF, IHC, WB
Human
Rabbit
Polyclonal
IgG
Peptide
Anti-MMP2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human MMP2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Liquid (sterile filtered)
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Invalid lot number

If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related products to: MMP2 Antibody