MECT1 Antibody
Anti-MECT1 Antibody - Western Blot
ELISA Results of Rabbit Anti-MECT1 Antibody
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Datasheet

MECT1 Antibody

Rabbit Polyclonal

600-401-936
100 µg
ELISA, WB, ChIP, IHC
Bovine, Zebrafish, Human, Mouse, Rat, Pufferfish
Rabbit
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$399.00 /Per Item
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. MECT1 (also known as Mucoepidermoid Carcinoma Translocated 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 Ser-133 phosphorylation. MECT1 enhances the interaction of CREB1 with TAF4. It regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Mect1 is a potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. MECT1 is also a coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, it is involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. MECT1 may be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, MECT1 regulates the light-induced entrainment of the circadian clock. In response to light stimulus, it coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. Therefore, it has been found to be important for energy balance and reproduction. Anti-MECT1 (CRTC1) Antibody is useful for researchers interested in glucose energy metabolism, circadian rhythms, and hormone research.

Application Note

This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80-95 kDa in size corresponding to MECT1 protein by western blotting in the appropriate cell lysate or extract.  This antibody is will react with isoform 1 and 2 of MECT1.

Purity/Specificity

This affinity-purified antibody is directed against human MECT1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross reactivity with MECT1 protein from human, mouse, rat, zebrafish, pufferfish and bovine based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.

Anti-MECT1 (RABBIT) Antibody - 600-401-936
rabbit anti-MECT1 antibody, MECT-1, MECT 1, TORC1, TORC-1, TORC 1, WAMTP1, WAMTP-1, CRTC1, CRTC-1, CREB-regulated transcription coactivator 1, Mucoepidermoid carcinoma translocated protein 1, Transducer of regulated cAMP response element-binding protein 1, Transducer of CREB protein 1
CRTC1
ELISA, WB
ChIP, IHC
Human
Bovine, Zebrafish, Human, Mouse, Rat, Pufferfish
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 19-34 of human MECT1 protein.
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Zhou X et al. Dependency of human and murine LKB1-inactivated lung cancer on aberrant CRTC-CREB activation. Elife. (2021)
Applications
WB, IB, PCA
Gu Y et al. Altered LKB1/CREB-regulated transcription co-activator (CRTC) signaling axis promotes esophageal cancer cell migration and invasion. Oncogene. (2012)
Applications
ChIP; IHC, ICC, Histology; WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.