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Datasheet

Laminin Antibody

Rabbit Polyclonal

2 References
600-401-116-0.1 600-401-116-0.5
100 µg 500 µg
WB, ELISA, IHC, Other
Human
Rabbit
$475.00 /Per Item
$949.00 /Per Item
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Background

Laminin Antibody is specific to Laminin. Laminins are major proteins in the basal lamina, a protein network foundation for most cells and organs. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. Anti-Laminin antibody is idea for investigators involved in cell cycle protein and extracellular matrix research.

Product Details

Anti-Laminin (RABBIT) Antibody - 600-401-116-0.1
rabbit anti-Laminin antibody, LAMA1 antibody, LAMB1 antibody, Laminin A chain antibody, Laminin alpha 1 antibody, Laminin alpha 1 chain antibody, Laminin B1 chain antibody, Laminin subunit beta 1 antibody
Rabbit
Polyclonal
IgG

Target Details

LAMA1/LAMB1  - View All LAMA1/LAMB1 Products
Human
Conjugated Peptide
Anti-Laminin Antibody was produced by repeated immunizations with a mixture of synthetic peptides corresponding to regions of adherence on laminin.
Anti-Laminin (RABBIT) Antibody has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities.  Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.  Anti-Laminin (Human) (RABBIT) Antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin.  Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.

Application Details

IHC
ELISA, Other, WB  - View References
Anti-Laminin (RABBIT) Antibody has been tested by IHC and was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5,000 to 1:10,000.  Anti-Laminin (Human) (RABBIT) Antibody was also assayed against 1.0 µg of Laminin in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.   A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product.  For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. Specific conditions should be optimized by researcher.

Formulation

Liquid (sterile filtered)
1.04 mg/mL by UV absorbance at 280 nm
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Expiration date is one (1) year from date of receipt.
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Applications
IHC, ICC, Histology
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Applications
E, EIA; Other; WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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