Lac I Antibody
Western Blot of Rabbit Anti-Lac I Antibody
Anti-Lac I Antibody - Western Blot
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Datasheet

Lac I Antibody

Rabbit Polyclonal

600-401-B04S 600-401-B04
25 µL 100 µg
ELISA, WB, IP
E. coli, Shigella sonnei
Rabbit
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$99.00 /Per Item
$399.00 /Per Item
25 µL $99.00
100 µg $399.00
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Description

Background

Anti-Lac I antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.

Application Note

Rabbit anti-Lac-I antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein.   Specific conditions for reactivity and detection of Lac I should be optimized by the end user.  Expect a band approximately ~38 kDa in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.

Purity/Specificity

This Anti-Lac I product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with recombinant Lac I protein.  A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence.  Cross-reactivity with Lac I from other sources has not been determined.

Anti-Lac I (RABBIT) Antibody - 600-401-B04
rabbit anti-LacI antibody, rabbit anti-Lac-I antibody, Rabbit anti-Lac I antibody, Lactose operon repressor, DNA binding transcriptional repressor antibody, Transcriptional repressor of the lac operon antibody
LacI
ELISA, WB
IP
Escherichia coli
E. coli, Shigella sonnei
Rabbit
Polyclonal
IgG
Recombinant Protein
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Wyatt et al. Novel Nonreplicating Vaccinia Virus Vector Enhances Expression of Heterologous Genes and Suppresses Synthesis of Endogenous Viral Proteins. mBio (2017)
Applications
WB, IB, PCA
Michel D et al. Kinetic approaches to lactose operon induction and bimodality. J Theor Biol. (2013)
Applications
IP, Co-IP; WB, IB, PCA
Agelopoulos M et al. Developmental regulation of chromatin conformation by Hox proteins in Drosophila. Cell Rep. (2012)
Applications
IP, Co-IP; WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.