IL-7 Receptor Alpha Chain phospho Y449 Antibody
Anti-IL-7 Receptor alpha chain pY449  - Western Blot
Western Blot - IL-7-Receptor-alpha-chain-pY449
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

IL-7 Receptor Alpha Chain phospho Y449 Antibody

Rabbit Polyclonal

600-401-A49
100 µg
ELISA, WB
Human, Mouse, Rat
Rabbit
Have a Question? Ask a Specialist
$399.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules.  The human and mouse sequence is nearly identical.  In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged. IL-7 Receptor Alpha Chain phospho Y449 Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Immunology research.

Application Note

This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 55 kDa in size corresponding to phosphorylated IL-7 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Purity/Specificity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against non-phospho-peptide. This antibody is specific for mouse IL-7 protein phosphorylated at Y449.  A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100% homology with the immunizing sequence.  Cross-reactivity with IL-7 from other sources has not been determined.

Anti-IL-7 Receptor alpha chain pY449 (RABBIT) Antibody - 600-401-A49
rabbit anti-IL-7 Receptor alpha pY449 antibody, rabbit anti-Interleukin-7 Receptor alpha pY449 antibody, IL7R alpha antibody, IL-7R alpha, IL 7R α, Interleukin7 Receptor, Interleukin 7 receptor alpha chain antibody, Interleukin 7 receptor α antibody
IL17R
ELISA, WB
Mouse
Human, Mouse, Rat
Phosphorylation
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
Liquid (sterile filtered)
0.72 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store IL-7 Receptor Alpha Chain phospho Y449 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Jaslow SL et al. The Salmonella Effector SarA Activates STAT3 to Promote an Anti-Inflammatory. Cell Rep. (2018)
Applications
WB, IB, PCA
Sugden S et al. HIV-1 tat protein recruits CIS to the cytoplasmic tail of CD127 to induce receptor ubiquitination and proteasomal degradation. Virology. (2016)
Applications
WB, IB, PCA

Invalid lot number

If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related products to: IL-7 Receptor Alpha Chain phospho Y449 Antibody