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Hsp70 Antibody

Mouse Monoclonal C92F3A-5 IgG1

3 References
200 µg
Human, Mouse, Rat, Bovine, C. elegans, Carp, Chicken, D. melanogaster, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep
$485.00 /Per Item
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Anti-Hsp 70 antibody is routinely used in cancer and signal transduction research. Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes, hsp70 genes exist as part of a multigene family. Hsp70s are found in most cellular compartments of eukaryotes, including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two-thirds of hsp70s are more conserved than the C-terminal one-third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins, preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization, and protein transport.

Product Details

Anti-HSP70 (MOUSE) Monoclonal Antibody - 200-301-A27
Heat shock 70 kDa protein 1 antibody, heat shock 70kDa protein 1A antibody, Heat shock 70kDa protein 1B antibody, Heat shock induced protein antibody, heat shock protein 70 antibody, HSP70 1 antibody

Target Details

HSPA1A  - View All HSPA1A Products
Human, Mouse, Rat, Bovine, C. elegans, Carp, Chicken, D. melanogaster, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep
Conjugated Peptide
Hsp70 monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide from the region of amino acid residues 436-503 of human Hsp70 protein.
This Protein G purified monoclonal antibody reacts with human and mouse Hsp70 protein.  A BLAST analysis was used to suggest cross-reactivity with Hsp70 from human, bovine, mouse, rat, C.elegans, dog, chicken, Drosophila, carp, guinea pig, hamster, monkey, pig, rabbit and sheep sources based on 100% homology with the immunizing sequence.  No cross-reactivity occurs with hsc70 (hsp73).  Cross-reactivity with Hsp70 from other sources has not been determined.

Application Details

Other  - View References
This Protein G purified antibody has been tested for use in ELISA, immunofluorescence microscopy, western blotting, FACS, immunoelectron microscopy, immunohistochemistry and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 70 kDa in size corresponding to Hsp70 by western blotting in the appropriate cell lysate or extract.  In general, a 1:1,000 dilution is suggested for most applications and is suitable to detect Hsp70 in 20 µg of heat shocked HeLa cell lysate by western blotting.


Liquid (sterile filtered)
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.1% (w/v) Sodium Azide
50% (v/v) Glycerol

Shipping & Handling

Dry Ice
Hsp70 antibody is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
(). USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila. Journal of Biological Chemistry
(). Nrf2 and HSF-1 Pathway Activation via Hydroquinone-Based Proelectrophilic Small Molecules is Regulated by Electrochemical Oxidation Potential. ASN Neuro
(). Dual neuroprotective pathways of a pro‐electrophilic compound via HSF‐1‐activated heat‐shock proteins and Nrf2‐activated phase 2 antioxidant response enzymes. J Neurochem.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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