Histone H2AvD phosphoS137 Antibody
Anti-Histone H2AvD pS137 Antibody - Immunohistochemistry
Anti-Histone H2AvD pS137 Antibody - Western Blot
Immunohistochemistry of Anti-Histone H2AvD pS137
Immunohistochemistry of Anti-Histone H2AvD pS137
Immunocytochemistry of Anti-Histone H2AvD pS137
Western Blot of Anti-Histone H2AvD pS137
Western Blot of Anti-Histone H2AvD pS137
Immunocytochemistry of Anti-Histone H2AvD pS137
Western Blot of Anti-Histone H2AvD pS137
Western Blot of Anti-Histone H2AvD pS137
Immunohistochemistry of Anti-Histone H2AvD pS137
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Datasheet

Histone H2AvD phosphoS137 Antibody

Rabbit Polyclonal

600-401-914S 600-401-914
25 µL 100 µg
ELISA, IHC, WB, EM, IF
D. melanogaster
Rabbit
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$99.00 /Per Item
$399.00 /Per Item
25 µL $99.00
100 µg $399.00
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known.  H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD  is expressed both maternally and zygotically and is found in embryos through to adults (female only).  The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur.  In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.

Application Note

Histone H2AvD pS137 Antibody is tested in ELISA, Immunohistochemistry, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract.  Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS137 of H2AvD protein.

Purity/Specificity

Affinity purified Anti-Histone H2AvD pS137 Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pS137 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against Drosophila H2AvD pS137 protein and the antibody is specific for the phosphorylated form of the protein.   Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA.  A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence.  Reactivity against homologues from other sources is not known.

Anti-Histone H2AvD pS137 (RABBIT) Antibody - 600-401-914
rabbit anti-H2AvD pS137 antibody, rabbit anti-Histone H2A.v pS137 antibody, H2AvD protein antibody, H2A.F/Z, H2A.Z, H2AvD, His2AvD, His2Av
His2Av
ELISA, IHC, WB
EM, IF
Drosophila melanogaster
D. melanogaster
Phosphorylation
Rabbit
Polyclonal
IgG
Peptide
Histone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 125-141 of Drosophila melanogaster (fruit fly) H2AvD protein.
Liquid (sterile filtered)
1.1 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Tu R et al. Multiple Niche Compartments Orchestrate Stepwise Germline Stem Cell Progeny Differentiation. Curr Biol. (2021)
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WB, IB, PCA
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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Undefined
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IF, Confocal Microscopy
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IF, Confocal Microscopy
Na HJ et al. Nutrient-driven O-GlcNAcylation controls DNA damage repair signaling and stem/progenitor cell homeostasis. Cell Rep. (2020)
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IHC, ICC, Histology
Lin KY et al. Piwi reduction in the aged niche eliminates germline stem cells via Toll-GSK3 signaling. Nat Commun. (2020)
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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IHC, ICC, Histology
Okumura K et al. Genetic identification and characterization of three genes that prevent accumulation of oxidative DNA damage in Drosophila adult tissues. DNA Repair (Amst). (2019)
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IF, Confocal Microscopy
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IF, Confocal Microscopy; WB, IB, PCA
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Applications
IF, Confocal Microscopy
Grendler J et al. Wound-induced polyploidization is driven by Myc and supports tissue repair in the presence of DNA damage. Development. (2019)
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IHC, ICC, Histology
Dialynas G et al. Arp2/3 and Unc45 maintain heterochromatin stability in Drosophila polytene chromosomes. Exp Biol Med (Maywood). (2019)
Applications
IF, Confocal Microscopy
Chang YH et al. The gypsy endogenous retrovirus drives non-cell-autonomous propagation in a Drosophila TDP-43 model of neurodegeneration. Curr Biol. (2019)
Applications
IF, Confocal Microscopy
Perlmutter JI et al. The phage gene wmk is a candidate for male killing by a bacterial endosymbiont. PLoS Pathog. (2019)
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IF, Confocal Microscopy
Nano M et al. Cell-cycle asynchrony generates DNA damage at mitotic entry in polyploid cells. Curr Biol. (2019)
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IF, Confocal Microscopy
Harumoto et al. Male-killing toxin in a bacterial symbiont of Drosophila. Nature (2018)
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IF, Confocal Microscopy
Mlih et al. A virus-acquired host cytokine controls systemic aging by antagonizing apoptosis. PLOS Biology (2018)
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IF, Confocal Microscopy
Merigliano et al. Protective role of vitamin B6 (PLP) against DNA damage in Drosophila models of type 2 diabetes. Scientific Reports (2018)
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IF, Confocal Microscopy
McCarthy et al. Tip60 complex promotes expression of a differentiation factor to regulate germline differentiation in female Drosophila. Molecular Biology of the Cell (2018)
Applications
IF, Confocal Microscopy
Feng L et al. Enhancer of polycomb maintains germline activity and genome integrity in Drosophila testis. Cell Death Differ. (2018)
Applications
IF, Confocal Microscopy
Clemot M et al. The replicative histone chaperone CAF1 is essential for the maintenance of identity and genome integrity in adult stem cells. Development. (2018)
Applications
IF, Confocal Microscopy
Caridi CP et al. Nuclear F-actin and myosins drive relocalization of heterochromatic breaks. Nature (2018)
Applications
WB, IB, PCA
Delabaere et al. Aging impairs double-strand break repair by homologous recombination in Drosophila germ cells. Aging Cell (2017)
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IF, Confocal Microscopy
Merigliano et al. A Role for the Twins Protein Phosphatase (PP2A-B55) in the Maintenance ofDrosophilaGenome Integrity. Genetics (2017)
Applications
IHC, ICC, Histology; WB, IB, PCA
Koehler et al. Pink1 and Parkin regulate Drosophila intestinal stem cell proliferation during stress and aging. Journal of Cell Biology (2017)
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IHC, ICC, Histology
Bayona-Feliu et al. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nature Communications (2017)
Applications
WB, IB, PCA
Ma X et al. Aubergine controls germline stem cell self-renewal and progeny differentiation via distinct mechanisms. Dev Cell. (2017)
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IHC, ICC, Histology
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Applications
IF, Confocal Microscopy
Colmenares SU et al. Drosophila histone demethylase KDM4A has enzymatic and non-enzymatic roles in controlling heterochromatin integrity. Dev Cell. (2017)
Applications
IF, Confocal Microscopy
Upadhyay et al. Transposon Dysregulation Modulates dWnt4 Signaling to Control Germline Stem Cell Differentiation in Drosophila. PLOS Genetics (2016)
Applications
WB, IB, PCA
Chen et al. Age-associated de-repression of retrotransposons in the Drosophila fat body, its potential cause and consequence. Aging Cell (2016)
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Immuno-EM
Ham et al. Glycine restores the anabolic response to leucine in a mouse model of acute inflammation. American Journal of Physiology-Endocrinology and Metabolism (2016)
Applications
WB, IB, PCA
Swenson et al. The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic. Elife (2016)
Applications
IHC, ICC, Histology
Hong et al. Antagonistic roles of Drosophila Tctp and Brahma in chromatin remodelling and stabilizing repeated sequences. Nature Communications (2016)
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IHC, ICC, Histology
Ma et al. DNA damage-induced Lok/CHK2 activation compromises germline stem cell self-renewal and lineage differentiation. Development (2016)
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IHC, ICC, Histology
Zaballos et al. The TALE Transcription Factor Homothorax Functions to Assemble Heterochromatin during Drosophila Embryogenesis. PLOS One (2015)
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IF, Confocal Microscopy
Ryu et al. Heterochromatic breaks move to the nuclear periphery to continue recombinational repair. Nature Cell Biology (2015)
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WB, IB, PCA
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Applications
IF, Confocal Microscopy
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IF, Confocal Microscopy
Hong ST et al. TCTP directly regulates ATM activity to control genome stability and organ development in Drosophila melanogaster. Nat Commun. (2013)
Applications
IHC, ICC, Histology
Chmielewski JP et al. Drosophila Psf2 has a role in chromosome condensation. Chromosoma. (2012)
Applications
IF, Confocal Microscopy
Vujatovic O, Zaragoza K, Vaquero A, Reina O, Bernués J, Azorín F. Drosophila melanogaster linker histone dH1 is required for transposon silencing and to preserve genome integrity. Nucleic Acids Res. (2012)
Applications
IF, Confocal Microscopy
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Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.