HIF-1-alpha hydroxy P564 Antibody
Anti-HIF-1alphaHYDROXYP564 Antibody - Western Blot
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Datasheet

HIF-1-alpha hydroxy P564 Antibody

Rabbit Polyclonal

100-401-A25
100 µL
ELISA, WB
Human
Rabbit
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$399.00 /Per Item
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Tumor hypoxia often directly correlates with aggressive phenotype, metastasis progression and resistance to chemotherapy. HIF-1 transcription factors are dramatically induced in hypoxic areas and regulate the expression of genes necessary for tumor adaptation to conditions of low oxygen. The stabilization of HIF-1a by hypoxia is critically dependent upon the hydroxylation of certain Proline residues that exist in the oxygen-dependent degradation domain of HIF-1a. HIF factors are now considered an important therapeutic target for cancer intervention. HIF-1a is useful to researchers interested in cell metabolism, cell survival, and angiogenesis.

Application Note

This antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 110 kDa in size corresponding to HIF-1a hydroxyl P564 by western blotting in the appropriate cell lysate or extract.

Purity/Specificity

This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein.  Minimal reactivity occurs with the non-hydroxylated form of the protein.  This antibody is specific for HIF-1a hydroxylated at P564.  Minimal cross-reactivity occurs with non-hydroxylated HIF-1a.  A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus sources based on a 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.

Anti-Hif-1α hydroxy P564 (RABBIT) Antibody - 100-401-A25
rabbit anti-Hif-1 alpha hydroxy P564 Antibody, Hypoxia-inducible factor 1-alpha antibody, ARNT-interacting protein antibody, Member of PAS protein 1 antibody, Basic-helix-loop-helix-PAS protein MOP1 antibody, PAS domain-containing protein 8 antibody, Class E basic helix-loop-helix protein 78 antibody
HIF1A
ELISA, WB
Human
Human
Hydroxylation
Rabbit
Polyclonal
Antiserum
Peptide
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1a.
Liquid (sterile filtered)
65 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Moorthy BT et al. The evolutionarily conserved arginyltransferase 1 mediates a pVHL-independent oxygen-sensing pathway in mammalian cells. Dev Cell. (2022)
Applications
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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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