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Datasheet

Hemoglobin A (beta chain) Antibody

Mouse Monoclonal 14G2.G11.F11 IgG2a lambda

3 References
200-301-GS4S 200-301-GS4
25 µL 100 µg
WB, ELISA, FC, LFA
Human
Mouse
$125.00 /Per Item
$485.00 /Per Item
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Background

HbA antibodies detect the hemoglobin beta subunit wild type variant A isoform. Functional adult hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 beta subunits (α2β2). Common isoform variants of hemoglobin include HbA, HbS, HbC, HbF, and HbA-2. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Globin gene mutations affect the structure and expression levels of Hb. Sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. Perhaps the most significant mutation is the E6V in the beta subunit and the cause of SCD, but other relevant isoforms of Hb are observed. HbA antibody cross reacts with HbA-2 but does not react other forms Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

Product Details

Anti-HbA (MOUSE) Monoclonal Antibody - 200-301-GS4
mouse anti-HbA antibody, mouse anti-hemoglobin antibody, Hemoglobin beta subunit, Hemoglobin subunit beta, Hemoglobin beta chain, HBB, HBA, Hbβ Antibody, LVV-hemorphin-7, Spinorphin, Sickle Cell Disease (SCD)
Mouse
Monoclonal
IgG2a

Target Details

HBB  - View All HBB Products
Human
Conjugated Peptide
Anti-Hemoglobin A (beta chain) Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb β-subunit conjugated to KLH.
This protein A purified mouse monoclonal antibody reacts specifically with human HbA beta chain isoform. Anti-Hemoglobin beta β is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, and Hylobates lar. This antibody does not react with the HbS, HbF (gamma), or HbC forms. HbA antibody cross reacts with HbA-2.

Application Details

ELISA, WB
FC, LFA  - View References
Anti-Hemoglobin A (beta chain) (MOUSE) antibody has been tested by ELISA and western blot. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.

Formulation

Liquid (sterile filtered)
1.04 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Reducing Child Mortality in Sierra Leone with a Sustainable Diagnostics Device for Sickle Cell Disease. 1st International Academic Conference on "WHY IT MATTERS”.
Applications
Lateral Flow (LFA)
(). Long-term outcomes of lentiviral gene therapy for the β-hemoglobinopathies: the HGB-205 trial. Nature Medicine
Applications
FC, FACS, FLOW
(). A Novel E-Junction Lateral Flow Immunoassay for Widespread Sickle Cell Screening in Low and Middle-Income Countries. IEEE Global Humanitarian Technology Conference (GHTC).
Applications
Lateral Flow (LFA)

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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