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Datasheet

GFP Monoclonal Antibody

Mouse Monoclonal 9F9.F9 IgG1 kappa

30 References
600-301-215S 600-301-215M 600-301-215
25 µL 100 µg 1 mg
Liquid (sterile filtered) Liquid (sterile filtered) Liquid (sterile filtered)
WB, ELISA, IHC, IF, FC, Dot Blot, IP, ChIP, Multiplex
GFP, eGFP, rGFP
Mouse
$125.00 /Per Item
$399.00 /Per Item
$829.00 /Per Item
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Background

Mouse anti-GFP antibody is functional by western blot, ELISA, Immunofluorescence Microscopy and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

Product Details

Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215
mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Mouse
Monoclonal
IgG

Target Details

GFP, eGFP, rGFP
Recombinant Protein
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215) from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.

Application Details

Dot Blot, ELISA, IP, WB
ChIP, FC, IF, IHC, Multiplex  - View References
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. Tested in ELISA, IP, and WB and suitable in FACS, IHC, IF. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store mouse anti-GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
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IHC, ICC, Histology
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FC, FACS, FLOW; WB, IB, PCA
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IF, Confocal Microscopy
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IP, Co-IP
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WB, IB, PCA
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ChIP
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WB, IB, PCA
(). The mono-ADP-ribosyltransferase ARTD10 regulates the voltage-gated K+ channel Kv1. 1 through protein kinase C delta. BMC Biol.
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WB, IB, PCA
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IF, Confocal Microscopy
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Applications
WB, IB, PCA
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IP, Co-IP
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TEM
(). Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope. Journal of Molecular Cell Biology
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WB, IB, PCA; IP, Co-IP
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WB, IB, PCA
(). Thalamostriatal and cerebellothalamic pathways in a songbird, the Bengalese finch. J Comp Neurol.
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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Applications
IF, Confocal Microscopy
(). Resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus: role of type I interferon signaling. Virology.
Applications
WB, IB, PCA
(). ARTD10 substrate identification on protein microarrays: regulation of GSK3β by mono-ADP-ribosylation. Cell Commun Signal.
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WB, IB, PCA
(). Recognition of mono-ADP-ribosylated ARTD10 substrates by ARTD8 macrodomains. Structure.
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WB, IB, PCA
(). Oncolytic vesicular stomatitis virus in an immunocompetent model of MUC1-positive or MUC1-null pancreatic ductal adenocarcinoma. J Virol.
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WB, IB, PCA
(). Long tethers provide high-force coupling of the Dam1 ring to shortening microtubules. Proc Natl Acad Sci U S A.
Applications
Bead Conjugation
(). Characterization of SLCO5A1/OATP5A1, a solute carrier transport protein with non-classical function. PloS One
Applications
WB, IB, PCA
(). Cochlin, Intraocular Pressure Regulation and Mechanosensing. PLoS One.
Applications
IHC, ICC, Histology; Multiplex Assay
(). Cochlin induced TREK-1 co-expression and annexin A2 secretion: role in trabecular meshwork cell elongation and motility. PLoS One.
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IHC, ICC, Histology; Multiplex Assay
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ChIP
(). ProMAT Calibrator: a tool for reducing experimental bias in antibody microarrays. J Proteome Res.
Applications
EIA, ELISA
(). Collagen Type I Induces disruption of E-cadherin–mediated cell-cell contacts and promotes proliferation of pancreatic carcinoma cells. Cancer Res.
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.