GFP Antibody
Anti-GFP Antibody - Immunofluorescence Microscopy
Anti-GFP Antibody - Western Blot
Western Blot - Anti-GFP Antibody
Western Blot Results of Chicken Anti-GFP Antibody
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Datasheet

GFP Antibody

Chicken Polyclonal

600-901-215S 600-901-215
25 µL 100 µg
Dot Blot, ELISA, WB, IF, IHC, Purification
eGFP, rGFP, WT
Chicken
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$99.00 /Per Item
$283.00 /Per Item
25 µL $99.00
100 µg $283.00
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Description

Background

Anti-GFP antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. GFP antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Chicken IgY lacks the classic "Fc" domain and does not bind to mammalian IgG Fc receptors. Thus resulting in lower backgrounds for western blotting, ELISA and Immunohistochemistry.

Application Note

Polyclonal anti-GFP is designed to detect GFP and its variants. Anti-GFP Antibody has been tested by LEISA, dot blot, and western and is suitable in immunofluorescence. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.

Purity/Specificity

Anti-GFP IgY was prepared from egg yolks by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).  No reaction was observed against Human, Mouse or Rat serum proteins.

Storage Condition

Store GFP Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-GFP (CHICKEN) Antibody - 600-901-215
chicken anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Dot Blot, ELISA, WB
IF, IHC, Purification
eGFP, rGFP, WT
GFP
Chicken
Polyclonal
IgG
Recombinant Protein
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Liquid (sterile filtered)
Dry Ice
Expiration date is one (1) year from date of opening.
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Lyu C et al. Building an allocentric travelling direction signal via vector computation. (2022)
Applications
IHC, ICC, Histology
Zhao X et al. 4D imaging analysis of the aging mouse neural stem cell niche reveals a dramatic loss of progenitor cell dynamism regulated by the RHO-ROCK pathway. (2022)
Applications
IHC, ICC, Histology
Oti T et al. Oxytocin influences male sexual activity via non-synaptic axonal release in the spinal cord. (2021)
Applications
Undefined
Osso LA et al. Experience-dependent myelination following stress is mediated by the neuropeptide dynorphin. (2021)
Applications
Undefined
Nam HS, Capecchi MR. Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life. (2020)
Applications
IF, Confocal Microscopy
Wu et al. let-7-Complex MicroRNAs Regulate Broad-Z3, Which Together with Chinmo Maintains Adult Lineage Neurons in an Immature State. (2020)
Applications
IF, Confocal Microscopy
Sanders DW et al. Competing protein-RNA interaction networks control multiphase intracellular organization. (2020)
Applications
Undefined
Duan ZRS et al. GABAergic restriction of network dynamics regulates interneuron survival in the developing cortex. (2020)
Applications
IHC, ICC, Histology
Goswami et al. Novel window for cancer nanotheranostics: non-invasive ocular assessments of tumor growth and nanotherapeutic treatment efficacy in vivo. (2019)
Applications
IHC, ICC, Histology
Chiu et al. Input-Specific NMDAR-Dependent Potentiation of Dendritic GABAergic Inhibition. (2018)
Applications
IF, Confocal Microscopy
Li et al. Hub-organized parallel circuits of central circadian pacemaker neurons for visual photoentrainment in Drosophila. (2018)
Applications
IF, Confocal Microscopy
Fujiwara Y et al. The CCHamide1 Neuropeptide Expressed in the Anterior Dorsal Neuron 1 Conveys a Circadian Signal to the Ventral Lateral Neurons in Drosophila melanogaster. (2018)
Applications
IHC, ICC, Histology
Kronenberg G et al. Distinguishing features of microglia-and monocyte-derived macrophages after stroke. (2018)
Applications
IHC, ICC, Histology
Oti T et al. Effects of sex steroids on the spinal gastrin-releasing peptide system controlling male sexual function in rats. (2018)
Applications
IF, Confocal Microscopy; WB, IB, PCA
Ribeiro IMA, Drews M, Bahl A, Machacek C, Borst A, Dickson BJ. Visual Projection Neurons Mediating Directed Courtship in Drosophila. (2018)
Applications
IF, Confocal Microscopy
Maruoka et al. Nectin-4 co-stimulates the prolactin receptor by interacting with SOCS1 and inhibiting its activity on the JAK2-STAT5a signaling pathway. (2017)
Applications
WB, IB, PCA
Neukomm LJ et al. Axon death pathways converge on axundead to promote functional and structural axon disassembly. (2017)
Applications
IF, Confocal Microscopy
Boye et al. Highly Efficient Delivery of Adeno-Associated Viral Vectors to the Primate Retina. (2016)
Applications
IHC, ICC, Histology
Chen et al. Lin-28 promotes symmetric stem cell division and drives adaptive growth in the adult Drosophila intestine. (2015)
Applications
IHC, ICC, Histology
He M. miRNA Tagging and Affinity-purification (miRAP). (2012)
Applications
IF, Confocal Microscopy
He M. miRNA Tagging and Affinity-purification (miRAP). (2012)
Applications
Ab Purification
Tseng C-Y, Firestein BL. The role of PSD-95 and cypin in morphological changes in dendrites following sublethal NMDA exposure. (2011)
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.