Cripto-1 TDGF1 Antibody
Anti-Cripto-1 Antibody - Western Blot
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Datasheet

Cripto-1 TDGF1 Antibody

Rabbit Polyclonal

600-401-997S 600-401-997
25 µL 100 µg
ELISA, WB, IHC
Human, Mouse
Rabbit
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$99.00 /Per Item
$399.00 /Per Item
25 µL $99.00
100 µg $399.00
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis.  During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor b (TGFb) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment.  In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation.  Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 21 kDa in size corresponding to Cripto-1 by western blotting in the appropriate cell lysate or extract.  Cripto-1 is reported as a 188 amino acid protein with a molecular weight of 21.1 kDa.

Purity/Specificity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human Cripto-1 protein.  A BLAST analysis was used to suggest limited cross-reactivity with Cripto-1 from mouse based on a 70% homology with the immunizing sequence.  Expect cross-reactivity with human Cripto-3 (TDGF2) based on very high levels of sequence conservation.  Cross-reactivity with Cripto-1 from other sources has not been determined.

Anti-Cripto-1 (TDGF1) (RABBIT) Antibody - 600-401-997
rabbit anti-Cripto 1 antibody, rabbit anti-Cripto-1 antibody, Cripto 1 growth factor antibody, Epidermal growth factor like cripto protein CR1 antibody, TDGF 1 antibody, Teratocarcinoma-derived growth factor 1, CRGF, TDGF1
TDGF1
ELISA, WB
IHC
Human
Human, Mouse
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Cripto-1 protein.
Liquid (sterile filtered)
0.97 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Gudbergsson JM et al. An evaluation of different Cripto‐1 antibodies and their variable results. J Cell Biochem. (2020)
Applications
IHC, ICC, Histology
Strizzi et al. Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi. Oncology Letters (2016)
Applications
IHC, ICC, Histology
Ligtenberg et al. Cripto-1 vaccination elicits protective immunity against metastatic melanoma. OncoImmunology (2016)
Applications
WB, IB, PCA
Tysnes BB et al. Age-dependent association between protein expression of the embryonic stem cell marker Cripto-1 and survival of glioblastoma patients. Transl Oncol. (2013)
Applications
IHC, ICC, Histology
Malchenko S et al. Cancer hallmarks in induced pluripotent cells: new insights. J Cell Physiol. (2010)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.