CEBP Delta Antibody
Anti-C/EBPd Antibody - Western Blot
Anti-C/EBPd Antibody - Western Blot
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Datasheet

CEBP Delta Antibody

Rabbit Polyclonal

600-401-A61S 600-401-A61
25 µL 100 µg
ELISA, WB, IHC
Mouse
Rabbit
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$99.00 /Per Item
$399.00 /Per Item
25 µL $99.00
100 µg $399.00
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.  Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression.  Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T).  Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity.  C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Application Note

This affinity purified antibody has been tested for use in ELISA, western blotting, and suitable in IHC.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35 kDa in size corresponding to C/EBPd by western blotting in the appropriate cell lysate or extract.   The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd.

Purity/Specificity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein.  A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 77% homology with the immunizing sequence.   No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with C/EBPd from other sources has not been determined.

Anti-C/EBP delta (RABBIT) Antibody - 600-401-A61
rabbit anti-CEBP Delta Antibody, C/EBP related protein 3 antibody, CCAAT/enhancer binding protein delta antibody, CEBP D antibody, CEBPD antibody, CELF antibody, Crp 3 antibody, Crp3 antibody, NF IL6 beta antibody, Nuclear factor NF IL6 beta antibody
Cebpd
ELISA, WB
IHC
Mouse
Mouse
Rabbit
Polyclonal
IgG
Recombinant Protein
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Valente T et al. CCAAT/enhancer binding protein δ is a transcriptional repressor of α-synuclein. Cell Death Differ. (2020)
Applications
IHC, ICC, Histology; IP, Co-IP; WB, IB, PCA
Sarkar TR et al. Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells. Mol Cell Biol. (2012)
Applications
WB, IB, PCA
Balamurugan K et al. The tumour suppressor C/EBPδ inhibits FBXW7 expression and promotes mammary tumour metastasis. EMBO J. (2010)
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.