Cbl-c Antibody
Anti-Cbl-c Antibody - Immunoprecipitation and Western Blot
Anti-Cbl-c Antibody – Immunohistochemistry
Anti-Cbl-c Antibody – Immunohistochemistry
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Datasheet

Cbl-c Antibody

Rabbit Polyclonal

600-401-889
100 µg
ELISA, IHC, IP, WB
Human
Rabbit
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$399.00 /Per Item
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cbl-c is also known as signal transduction protein CBL-C, SH3-binding protein CBL-C, CBL-3, and RING finger protein 57.  Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation.  Cbl-c (a.k.a. Cbl-3) is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed).  Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation.  Cbl-c knock out mice show no obvious phenotype.  Thus, the physiological role of Cbl-c is not known.

Application Note

This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, immunoprecipitation and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~52 kDa in size corresponding to Cbl-c by western blotting in the appropriate cell lysate or extract.

Purity/Specificity

This affinity purified antibody is directed against human Cbl-c protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest that this antibody would react with Cbl-c from human and chimpanzee sources.  Expect partial reactivity against mouse and rat sources of Cbl-c as ~83% sequence homology is on record for the immunogen sequence.  Reactivity with Cbl-c from other sources has not been determined.  No reactivity is expected with Cbl-a or Cbl-b.

Anti-cbl-c (RABBIT) Antibody - 600-401-889
rabbit anti-Cbl-c Antibody, E3 ubiquitin-protein ligase CBL-C, RING finger protein 57, RING-type E3 ubiquitin transferase CBL-C, SH3-binding protein CBL-3, SH3-binding protein CBL-C, Signal transduction protein CBL-C, CBLC, CBL3, RNF57
CBLC
ELISA, IHC, IP, WB
Human
Human
Rabbit
Polyclonal
IgG
Peptide
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal portion of Human Cbl-c.
Liquid (sterile filtered)
1.2 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Hong SY et al. Stabilization of AURKA by the E3 ubiquitin ligase CBLC in lung adenocarcinoma. Oncogene. (2022)
Applications
Undefined
Hong SY et al. Upregulation of E3 Ubiquitin Ligase CBLC Enhances EGFR Dysregulation and Signaling in Lung Adenocarcinoma. Cancer Res. (2018)
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.