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ATM phospho S1981 Antibody
Mouse Monoclonal 10H11.E12 IgG1 kappa
200-301-400S
200-301-400
25 µL
100 µg
ELISA, FC, IF, SDS-PAGE, WB, Biochemical Assay, ChIP, FISH, IHC, IP
Human, Mouse, Rat
Mouse
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Availability: Ships next business day
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Description
Background
Anti ATM pS1981 Antibody is a phospho site specific antibody and recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370-kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint; Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest; and Brca1 and hRad17 in the G2/M checkpoint. Ideal for Cancer, Cell Signaling, Chromatin, Neuroscience and Signal Transduction research.
Application Note
Protein A Purified Mab anti-ATM has been tested by ELISA, Flow Cytometry, IF, SDS-PAGE, and western blotting against both the native and recombinant forms of the protein. The antibody immunoprecipitates ATM from irradiated human and transfected mouse cells. By immunofluorescence, foci are detected in irradiated human and mouse fibroblasts. This antibody is not recommended for immunohistochemistry. Instead, for IHC, use the clone 7C10D8 (p/n 200-301-500).
Purity/Specificity
Anti-ATM phospho S1981 Monoclonal Antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. By ELISA reactivity against SLAFEEGSpQSTTISS at a 1:1600 dilution shows an absorbance >3.000; whereas reactivity against SLAFEEGSQSTTISS shows and absorbance of 0.145. Reactivity is observed against human ATM. Cross reactivity with ATM from other mammalian sources has not been tested. The immunogen has 91% sequence homology with mouse ATM.
Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody - 200-301-400
mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody
ATM
ELISA, FC, IF, SDS-PAGE, WB
Biochemical Assay, ChIP, FISH, IHC, IP
Human, Mouse, Rat
Mouse
Phosphorylation
Monoclonal
10H11.E12
IgG1
Peptide
Anti-ATM phospho S1981 Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store Anti-ATM phospho S1981 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Montales, K et al. MRN-dependent and independent pathways for recruitment of TOPBP1 to DNA double-strand breaks. PloS One (2022)
Applications
WB, IB, PCA
Ishimoto R et al. SLX4–XPF mediates DNA damage responses to replication stress induced by DNA–protein interactions. J Cell Biol (2021)
Applications
Undefined
Cabrini M et al. DROSHA is recruited to DNA damage sites by the MRN complex to promote non-homologous end-joining. J Cell Biol (2021)
Applications
ChIP; IF, Confocal Microscopy
Montales, K et al. Structure-function analysis of TOPBP1's role in ATR signaling using the DSB-mediated ATR activation in Xenopus egg extracts (DMAX) system. Scientific Reports (2021)
Applications
WB, IB, PCA
Tao Y et al. Inhibition of DNA replication initiation by silver nanoclusters. Nucleic Acids Res. (2021)
Applications
Undefined
Qi Y et al. Pb induced mitochondrial fission of fibroblast cells via ATM activation. J Hazard Mater. (2021)
Applications
Undefined
La NL et al. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer. Cell Rep. (2021)
Applications
WB, IB, PCA
Herok M et al. Chemotherapy of HER2- and MDM2-Enriched Breast Cancer Subtypes Induces Homologous Recombination DNA Repair and Chemoresistance. Cancers (Basel). (2021)
Applications
WB, IB, PCA
Homma H et al. DNA damage in embryonic neural stem cell determines FTLDs' fate via early-stage neuronal necrosis. Life Sci Alliance. (2021)
Applications
WB, IB, PCA
Wang YL et al. Evading immune surveillance via tyrosine phosphorylation of nuclear PCNA. Cell Rep. (2021)
Applications
WB, IB, PCA
Nguyen A et al. Class 1 Histone Deacetylases and Ataxia-Telangiectasia Mutated Kinase Control the Survival of Murine Pancreatic Cancer Cells upon dNTP Depletion. Cells. (2021)
Applications
WB, IB, PCA
Tanaka H et al. HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology. Communications Biology (2021)
Applications
WB, IB, PCA
Hayashi K et al. Association of glomerular DNA damage and DNA methylation with one-year eGFR decline in IgA nephropathy. Sci Rep. (2020)
Applications
IHC, ICC, Histology
Valdez BC et al. Panobinostat and venetoclax enhance the cytotoxicity of gemcitabine, busulfan, and melphalan in multiple myeloma cells. Exp Hematol (2020)
Applications
WB, IB, PCA
Zhu C et al. Phospho-Ser784-VCP is required for DNA damage response and is associated with poor prognosis of chemotherapy-treated breast cancer. Cell Rep (2020)
Applications
IF, Confocal Microscopy
Zhai B et al. Dianhydrogalactitol synergizes with topoisomerase poisons to overcome DNA repair activity in tumor cells. Cell Death Dis (2020)
Applications
WB, IB, PCA
Deville SS et al. Keap1 inhibition sensitizes head and neck squamous cell carcinoma cells to ionizing radiation via impaired non-homologous end joining and induced autophagy. Cell Death Dis (2020)
Applications
Kinase Assay; WB, IB, PCA
Zhoa J et al. ATM is a key driver of NF-κB-dependent DNA-damage-induced senescence, stem cell dysfunction and aging. Aging (Albany NY) (2020)
Applications
WB, IB, PCA
Cui D et al. FBXW7 confers radiation survival by targeting p53 for degradation. Cell Rep (2020)
Applications
WB, IB, PCA
Pan, YR et al. ATM Inhibitor Suppresses Gemcitabine-Resistant BTC Growth in a Polymerase θ Deficiency-Dependent Manner. Biomolecules (2020)
Applications
IF, Confocal Microscopy
Shu, J et al. SCFβ-TrCP-mediated degradation of TOP2β promotes cancer cell survival in response to chemotherapeutic drugs targeting topoisomerase II. Oncogenesis (2020)
Applications
WB, IB, PCA
Choi C et al. Targeting DNA-dependent protein kinase sensitizes hepatocellular carcinoma cells to proton beam irradiation through apoptosis induction. PLoS One (2019)
Applications
WB, IB, PCA
Jin S et al. ATM controls DNA repair and mitochondria transfer between neighboring cells. Cell Commun Signal (2019)
Applications
IF, Confocal Microscopy; WB, IB, PCA
Crochemore C et al. CSB promoter downregulation via histone H3 hypoacetylation is an early determinant of replicative senescence. Nat Commun (2019)
Applications
WB, IB, PCA
Li et al. PPARγ Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis. Cell Reports (2019)
Applications
IF, Confocal Microscopy; WB, IB, PCA
Kreis et al. ATM phosphorylation of the actin-binding protein drebrin controls oxidation stress-resistance in mammalian neurons and C. elegans. Nature Communications (2019)
Applications
IHC, ICC, Histology
Johnson, DP et al. Assessment of epigenetic mechanisms and DNA double-strand break repair using laser micro-irradiation technique developed for hematological cells. EbioMedicine (2019)
Applications
IF, Confocal Microscopy
Gu X et al. Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway. Toxicol Lett (2018)
Applications
Undefined
Valdez BC et al. Epigenetic modification enhances the cytotoxicity of busulfan and4-hydroperoxycyclophosphamide in AML cells. Exp Hematol (2018)
Applications
WB, IB, PCA
Gupta R et al. DNA repair network analysis reveals shieldin as a key regulator of NHEJ and PARP inhibitor sensitivity. Cell. (2018)
Applications
WB, IB, PCA
Sun J et al. Distinct roles of ATM and ATR in the regulation of ARP8 phosphorylation to prevent chromosome translocations. Elife (2018)
Applications
WB, IB, PCA
Evangelista FM et al. Transcription and mRNA export machineries SAGA and TREX-2 maintain monoubiquitinated H2B balance required for DNA repair. J Cell Biol (2018)
Applications
WB, IB, PCA
Chen et al. The Augmented R-Loop Is a Unifying Mechanism for Myelodysplastic Syndromes Induced by High-Risk Splicing Factor Mutations. Molecular Cell (2018)
Applications
IF, Confocal Microscopy; WB, IB, PCA
Göder et al. HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130. Nature Communications (2018)
Applications
IP, Co-IP; WB, IB, PCA
Vadnais et al. GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1. Nature Communications (2018)
Applications
FC, FACS, FLOW; IF, Confocal Microscopy
Zhai et al. Dianhydrogalactitol induces replication-dependent DNA damage in tumor cells preferentially resolved by homologous recombination. Cell Death & Disease (2018)
Applications
WB, IB, PCA
Fujiwara et al. Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells. Scientific Reports (2018)
Applications
IF, Confocal Microscopy; ISH, FISH
Parisotto et al. PTEN deletion in luminal cells of mature prostate induces replication stress and senescence in vivo. Journal of Experimental Medicine (2018)
Applications
IHC, ICC, Histology
Zheng et al. Oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation. Molecular Medicine Reports (2017)
Applications
IF, Confocal Microscopy; WB, IB, PCA
Ashraf et al. Coumarin-chalcone hybrid instigates DNA damage by minor groove binding and stabilizes p53 through post translational modifications. Scientific Reports (2017)
Applications
WB, IB, PCA
Valdez et al. The PARP inhibitor olaparib enhances the cytotoxicity of combined gemcitabine, busulfan and melphalan in lymphoma cells. Leukemia & Lymphoma (2017)
Applications
WB, IB, PCA
Higo et al. DNA single-strand break-induced DNA damage response causes heart failure. Nature Communications (2017)
Applications
WB, IB, PCA
Gursoy-Yuzugullu et al. Epigenetic therapy with inhibitors of histone methylation suppresses DNA damage signaling and increases glioma cell radiosensitivity. Oncotarget (2017)
Applications
IHC, ICC, Histology
Feng et al. BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination. Nature Communications (2017)
Applications
IF, Confocal Microscopy
Chen et al. LRRK2 interacts with ATM and regulates Mdm2-p53 cell proliferation axis in response to genotoxic stress. Human Molecular Genetics (2017)
Applications
WB, IB, PCA
Michelini et al. Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. Nature Cell Biology (2017)
Applications
IF, Confocal Microscopy
Dubois EL et al. UV-dependent phosphorylation of COP9/signalosome in UV-induced apoptosis. Oncol Rep (2016)
Applications
WB, IB, PCA
Qi et al. ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts. Scientific Reports (2016)
Applications
IF, Confocal Microscopy
Stankovic-Valentin et al. Redox regulation of SUMO enzymes is required for ATM activity and survival in oxidative stress. The EMBO Journal (2016)
Applications
WB, IB, PCA
Inoue et al. Mutant IDH1 Downregulates ATM and Alters DNA Repair and Sensitivity to DNA Damage Independent of TET2. Cancer Cell (2016)
Applications
WB, IB, PCA
Bakr et al. Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Oncotarget (2016)
Applications
IF, Confocal Microscopy
Hansen et al. SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nature Cell Biology (2016)
Applications
IF, Confocal Microscopy; WB, IB, PCA
Xue L et al. The complexity of DNA double strand break is a crucial factor for activating ATR signaling pathway for G2/M checkpoint regulation regardless of ATM function. DNA Repair (Amst) (2015)
Applications
Undefined
Huskey NE et al. CDK1 inhibition targets the p53-NOXA-MCL1 axis, selectively kills embryonic stem cells, and prevents teratoma formation. Stem Cell Reports (2015)
Applications
WB, IB, PCA
Vermezovic J et al. Notch is a direct negative regulator of the DNA-damage response. Nat Struct Mol Biol (2015)
Applications
Undefined
Valdez et al. Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators. Experimental Hematology (2015)
Applications
WB, IB, PCA
Xiong et al. Stemness factor Sall4 is required for DNA damage response in embryonic stem cells. Journal of Cell Biology (2015)
Applications
IF, Confocal Microscopy
Modzelewski et al. Dgcr8 and Dicer are essential for sex chromosome integrity during meiosis in males. Journal of Cell Science (2015)
Applications
IF, Confocal Microscopy
Wang'ondu et al. DNA Damage Signaling Is Induced in the Absence of Epstein-Barr Virus (EBV) Lytic DNA Replication and in Response to Expression of ZEBRA. PLOS One (2015)
Applications
IF, Confocal Microscopy
Hartlerode et al. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nature Structural & Molecular Biology (2015)
Applications
WB, IB, PCA
Torres et al. Role of the Exocyst Complex Component Sec6/8 in Genomic Stability. Molecular and Cellular Biology (2015)
Applications
WB, IB, PCA
Sifford et al. Murine Gammaherpesvirus 68 LANA and SOX Homologs Counteract ATM-Driven p53 Activity during Lytic Viral Replication. Journal of Virology (2015)
Applications
WB, IB, PCA
Broering TJ et al. BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis. J Cell Biol (2014)
Applications
IF, Confocal Microscopy; IP, Co-IP
Manguan-Garcia C et al. Expression of the genetic suppressor element 24.2 (GSE24. 2) decreases DNA damage and oxidative stress in X-linked dyskeratosis congenita cells. PLoS One (2014)
Applications
IF, Confocal Microscopy
Smith CL et al. A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli. Mol Biol Cell (2014)
Applications
WB, IB, PCA
Fumagalli M et al. Stable cellular senescence is associated with persistent DDR activation. PLoS One (2014)
Applications
IF, Confocal Microscopy
Carvalho, S et al. SETD2 is required for DNA double-strand break repair and activation of the p53-mediated checkpoint. ELife (2014)
Applications
WB, IB, PCA
Wu L et al. BMS-345541 sensitizes MCF-7 breast cancer cells to ionizing radiation by selective inhibition of homologous recombinational repair of DNA double-strand breaks. Radiat Res (2013)
Applications
IF, Confocal Microscopy
Castro-Vega LJ et al. Telomere crisis in kidney epithelial cells promotes the acquisition of a microRNA signature retrieved in aggressive renal cell carcinomas. Carcinogenesis (2013)
Applications
WB, IB, PCA
Birkelbach M et al. Detection of impaired homologous recombination repair in NSCLC cells and tissues. J Thorac Oncol (2013)
Applications
IF, Confocal Microscopy
Floyd SR et al. The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. Nature (2013)
Applications
Undefined
Shamma A et al. ATM mediates pRB function to control DNMT1 protein stability and DNA methylation. Mol Cell Biol (2013)
Applications
IF, Confocal Microscopy; WB, IB, PCA
Min W et al. Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation. Nat Commun (2013)
Applications
IP, Co-IP; WB, IB, PCA
Cesare AJ et al. The telomere deprotection response is functionally distinct from the genomic DNA damage response. Mol Cell. (2013)
Applications
WB, IB, PCA
Lim HC et al. Ribosomal S6 Kinase 2 (RSK2) maintains genomic stability by activating the Atm/p53-dependent DNA damage pathway. PLoS One (2013)
Applications
WB, IB, PCA
Toledo LI et al. ATR prohibits replication catastrophe by preventing global exhaustion of RPA. Cell. (2013)
Applications
WB, IB, PCA
Rodriguez-Hernandez C.J. et al. Sodium tungstate modulates ATM function upon DNA damage. FEBS Lett. (2013)
Applications
WB, IB, PCA
Vidal-Eychenié S, Décaillet C, Basbous J, Constantinou A. DNA structure-specific priming of ATR activation by DNA-PKcs. J Cell Biol. (2013)
Applications
WB, IB, PCA
Vogel R et al. Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection. J Virol (2012)
Applications
IF, Confocal Microscopy
Daugherity EK et al. The DNA damage checkpoint protein ATM promotes hepatocellular apoptosis and fibrosis in a mouse model of non-alcoholic fatty liver disease. Cell Cycle (2012)
Applications
IF, Confocal Microscopy
Vadnais C et al. CUX1 transcription factor is required for optimal ATM/ATR-mediated responses to DNA damage. Nucleic Acids Res (2012)
Applications
WB, IB, PCA
Fumagalli M et al. Telomeric DNA damage is irreparable and causes persistent DNA-damage-response activation. Nat Cell Biol (2012)
Applications
IF, Confocal Microscopy
Javvadi P et al. Threonine 2609 phosphorylation of the DNA-dependent protein kinase is a critical prerequisite for epidermal growth factor receptor-mediated radiation resistance. Mol Cancer Res (2012)
Applications
IP, Co-IP; WB, IB, PCA
Osorio FG et al. Nuclear lamina defects cause ATM-dependent NF-κB activation and link accelerated aging to a systemic inflammatory response. Genes Dev. (2012)
Applications
WB, IB, PCA
Juardo S et al. Novel function of the DNA damage response protein ASCIZ as an essential transcription factor. J Biol Chem (2012)
Applications
Undefined
Hayashi MT et al. A telomere-dependent DNA damage checkpoint induced by prolonged mitotic arrest. Nat Struct Mol Biol (2012)
Applications
WB, IB, PCA
Daugaard M et al. LEDGF (p75) promotes DNA-end resection and homologous recombination. Nat Struct Mol Biol (2012)
Applications
WB, IB, PCA
Gudjonsson T et al. TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at damaged chromosomes. Cell (2012)
Applications
WB, IB, PCA
Suzuki K et al. Creating localized DNA double-strand breaks with microirradiation. Nat Protoc (2011)
Applications
Undefined
Ling S et al. EDD inhibits ATM-mediated phosphorylation of p53. J Biol Chem (2011)
Applications
WB, IB, PCA
Sugimoto N et al. Chromatin remodeler sucrose nonfermenting 2 homolog (SNF2H) is recruited onto DNA replication origins through interaction with Cdc10 protein-dependent transcript 1 (Cdt1) and promotes pre-replication complex formation. J Biol Chem (2011)
Applications
WB, IB, PCA
Al Rashid ST et al. Protein-protein interactions occur between p53 phosphoforms and ATM and 53BP1 at sites of exogenous DNA damage. Radiat Res (2011)
Applications
Undefined
Fradet-Turcotte A et al. Nuclear accumulation of the papillomavirus E1 helicase blocks S-phase progression and triggers an ATM-dependent DNA damage response. J Virol. (2011)
Applications
IF, Confocal Microscopy
Zhang W, Peng G, Lin SY, Zhang P. DNA damage response is suppressed by the high cyclin-dependent kinase 1 activity in mitotic mammalian cells. J Biol Chem. (2011)
Applications
WB, IB, PCA
Boichuk S et al. Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen. J Virol (2010)
Applications
IF, Confocal Microscopy
Jun JI et al. The matricellular protein CCN1 induces fibroblast senescence and restricts fibrosis in cutaneous wound healing. Nat Cell Biol (2010)
Applications
WB, IB, PCA
Sato K et al. γ-H2AX and phosphorylated ATM focus formation in cancer cells after laser plasma X irradiation. Radiat Res (2010)
Applications
Undefined
Larsen DH et al. The chromatin-remodeling factor CHD4 coordinates signaling and repair after DNA damage. J Cell Biol (2010)
Applications
Undefined
Hurov KE et al. A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability. Genes Dev. (2010)
Applications
WB, IB, PCA
Juardo S et al. Dual functions of ASCIZ in the DNA base damage response and pulmonary organogenesis. PLoS One (2010)
Applications
WB, IB, PCA
Alimonti A et al. A novel type of cellular senescence that can be enhanced in mouse models and human tumor xenografts to suppress prostate tumorigenesis. J Clin Invest (2010)
Applications
IF, Confocal Microscopy
Adeyemi RO et al. Parvovirus minute virus of mice induces a DNA damage response that facilitates viral replication. Plos Pathog (2010)
Applications
IF, Confocal Microscopy
Sugimoto N et al. Redundant and differential regulation of multiple licensing factors ensures prevention of re-replication in normal human cells. J Cell Sci (2009)
Applications
WB, IB, PCA
Efeyan A. et al. Limited role of murine ATM in oncogene-induced senescence and p53-dependent tumor suppression. PLoS One (2009)
Applications
WB, IB, PCA
Collaco RF et al. Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways. Virology (2009)
Applications
WB, IB, PCA
Acota JC et al. Chemokine signaling via the CXCR2 receptor reinforces senescence. Cell (2008)
Applications
IF, Confocal Microscopy
Sugimoto N et al. Identification of Novel Human Cdt1-binding Proteins by a Proteomics Approach: Proteolytic Regulation by APC/CCdh1. Mol Biol Cell (2008)
Applications
WB, IB, PCA
Ramirez T et al. Prevention of aneuploidy by S-adenosyl-methionine in human cells treated with sodium arsenite. Mutat Res (2007)
Applications
Undefined
Jacquemont C et al. Proteasome function is required for DNA damage response and fanconi anemia pathway activation. Cancer Res. (2007)
Applications
IF, Confocal Microscopy
Acosta JC et al. Tip60 is a haplo-insufficient tumour suppressor required for an oncogene-induced DNA damage response. Nature (2007)
Applications
IF, Confocal Microscopy
Pusapati RV, Rounbehler RJ, Hong S, et al. ATM promotes apoptosis and suppresses tumorigenesis in response to Myc. Proc Natl Acad Sci U S A. (2006)
Applications
WB, IB, PCA
Warters RL et al. Melanoma cells express elevated levels of phosphorylated histone H2AX foci. J Invest Dermatol (2005)
Applications
Undefined
Falck J, Coates J, Jackson SP. Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature (2005)
Applications
IF, Confocal Microscopy
Bartkova J, Horejsí Z, Koed K, et al. DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature (2005)
Applications
IF, Confocal Microscopy; IHC, ICC, Histology
Bartkova J, Bakkenist CJ, Rajpert-De Meyts E, et al. ATM activation in normal human tissues and testicular cancer. Cell Cycle (2005)
Applications
IP, Co-IP; WB, IB, PCA; IHC, ICC, Histology
Powers JT et al. E2F1 uses the ATM signaling pathway to induce p53 and Chk2 phosphorylation and apoptosis. Mol Cancer Res. (2004)
Applications
WB, IB, PCA
Kitagawa et al. Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. (2004)
Applications
WB, IB, PCA; IF, Confocal Microscopy
Bakkenist CJ, Kastan MB. DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature (2003)
Applications
IP, Co-IP; WB, IB, PCA; IF, Confocal Microscopy
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.