Anti-mCherry Antibody Pre-Adsorbed
Western Blot of Rabbit Anti-mCherry Antibody
Western Blot of Anti-mCherry Antibody
Western Blot -Rabbit anti-mCherry
Western Blot of Anti-mCherry
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Datasheet

Anti-mCherry Antibody Pre-Adsorbed

Rabbit Polyclonal

22 References
600-401-P16S 600-401-P16
25 µL 100 µg
WB, ELISA, IHC, IF, IP, Multiplex
RFP, mCherry, rRFP
Rabbit
$125.00 /Per Item
$285.00 /Per Item
25 µL $125.00
100 µg $285.00
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Background

mCherry Antibody is ideal for Western Blotting. Fluorescent proteins such as Discosoma Red Fluorescent Protein (and its variants), and GFP are widely used in research practice. Both commonly serve as a markers for gene expression and protein localization. DsRed was isolated from sea anemone Discosoma sp. mushroom and GFP is originated from Aequorea victoria jellyfish. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional steps in the chromophore maturation and obligates tetrameric structure. All mCherry antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein.

Product Details

Anti-mCherry (RABBIT) Antibody (Min X Hu Ms & Rt Serum Proteins) - 600-401-P16
rabbit anti-mCherry antibody, RFP, mCherry monomeric red fluorescent protein, Red Fluorescent Protein (RFP), rDsRed, Discosoma sp. Red Fluorescent Protein, mRFP1, mBanana, mHoneydew, mPlum, mOrange, mStrawberry, mTangerine
Rabbit
Polyclonal
IgG

Target Details

DsRed
RFP, mCherry, rRFP
The immunogen is a mCherry mutant variant fusion protein of RFP corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
mCherry was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Expect reactivity against mCherry, RFP and its variants: tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified mCherry.  No reaction was observed against Human, Mouse or Rat serum proteins.  ELISA was used to confirm specificity at less than 0.1% of target signal.

Application Details

ELISA, WB
IF, IHC, IP, Multiplex  - View References
Polyclonal anti-mCherry is designed to detect mCherry, RFP, and its variants. Anti-mCherry (Discosoma sp.) has been tested by ELISA and Western blot and is intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS). Researchers should determine optimal titers for applications that are not stated. In addition, we performed conjugation of RFP antibodies to either fluorescent dyes, biotin or horseradish peroxidase to further facilitate RFP protein detection and quantification.

Formulation

Liquid (sterile filtered)
1.17 by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide

Shipping & Handling

Dry Ice
Store mCherry Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Lifelong single-cell profiling of cranial neural crest diversification in zebrafish. Nature Communications
Applications
IHC, ICC, Histology
(). New look at RSV infection: tissue clearing and 3D imaging of the entire mouse lung at cellular resolution. Viruses.
Applications
IHC, ICC, Histology
(). Pharmacogenetic inhibition of lumbosacral sensory neurons alleviates visceral hypersensitivity in a mouse model of chronic pelvic pain. PLoS One.
Applications
IHC, ICC, Histology
(). DYNC1LI2 regulates localization of the chaperone-mediated autophagy receptor LAMP2A and improves cellular homeostasis in cystinosis. Autophagy.
Applications
WB, IB, PCA
(). A Dominant Heterozygous Mutation in COG4 Causes Saul–Wilson Syndrome, a Primordial Dwarfism, and Disrupts Zebrafish Development via Wnt Signaling. Front Cell Dev Biol.
Applications
WB, IB, PCA
(). 14-3-3 proteins promote synaptic localization of N-methyl d-aspartate receptors (NMDARs) in mouse hippocampal and cortical neurons. PLoS One.
Applications
WB, IB, PCA
(). Neurochemical Characterization of Brainstem Pro-Opiomelanocortin Cells. Endocrinology
Applications
IF, Confocal Microscopy
(). Respiratory syncytial virus tropism for olfactory sensory neurons in mice. J Neurochem.
Applications
IF, Confocal Microscopy
(). Histological assessment of optogenetic tools to study fronto-visual and fronto-parietal cortical networks in the rhesus macaque. Sci Rep.
Applications
IF, Confocal Microscopy; IHC, ICC, Histology; Multiplex Assay
(). A new reporter zebrafish line unveils heterogeneity among lymphatic endothelial cells during development. Dev Dyn.
Applications
Undefined
(). Patient-specific genomics and cross-species functional analysis implicate LRP2 in hypoplastic left heart syndrome. ELife
Applications
IF, Confocal Microscopy
(). The nucleotide sensor ZBP1 and kinase RIPK3 induce the enzyme IRG1 to promote an antiviral metabolic state in neurons. Immunity.
Applications
IF, Confocal Microscopy; Multiplex Assay
(). Imaging of morphological and biochemical hallmarks of apoptosis with optimized optogenetic tools. J Biol Chem.
Applications
WB, IB, PCA
(). Translational regulation promotes oxidative stress resistance in the human fungal pathogen cryptococcus neoformans. mBio.
Applications
WB, IB, PCA
(). Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity. PLOS Pathogens
Applications
IP, Co-IP; WB, IB, PCA
(). Genetically engineered rat gliomas: PDGF-driven tumor initiation and progression in tv-a transgenic rats recreate key features of human brain cancer. PLOS One
Applications
IHC, ICC, Histology
(). RIPK3 Restricts Viral Pathogenesis via Cell Death-Independent Neuroinflammation. Cell
Applications
IHC, ICC, Histology
(). Inferior Olivary TMEM16B Mediates Cerebellar Motor Learning. Neuron
Applications
IF, Confocal Microscopy; Multiplex Assay
(). Pbx4 is Required for the Temporal Onset of Zebrafish Myocardial Differentiation. The International Journal of Developmental Biology
Applications
IF, Confocal Microscopy; Multiplex Assay
(). Expression of active fluorophore proteins in the milk of transgenic pigs bypassing the secretory pathway. Sci Rep.
Applications
WB, IB, PCA
(). SQSTM1 splice site mutation in distal myopathy with rimmed vacuoles. Neurology
Applications
WB, IB, PCA
(). Adult neurogenesis produces neurons with unique GABAergic synapses in the olfactory bulb. The Journal of Neuroscience
Applications
IHC, ICC, Histology; Multiplex Assay

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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