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Datasheet

Alpha-1-Anti-Trypsin Antibody Biotin Conjugated

Goat Polyclonal

200-106-147 200-106-147S 200-106-147-0100
10 mg 25 µL 100 µg
Lyophilized Liquid (sterile filtered) Lyophilized
WB, ELISA
Human
Goat
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$455.00 /Per Item
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Product Details

Anti-Alpha-1-Anti-Trypsin (Human Plasma) (GOAT) Antibody Biotin Conjugated (BULK ORDER) - 200-106-147
goat anti-Alpha-1-Trypsin Antibody biotin Conjugation, biotin Conjugated goat anti-Alpha-1-Trypsin Antibody, A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibody
Goat
Biotin
Polyclonal
IgG

Target Details

SERPINA1  - View All SERPINA1 Products
Human
Native Protein
a1-Anti-Trypsin [Human Plasma]
Anti-Alpha-1-Anti-Trypsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified a-1-anti-Trypsin [Human Plasma].  Cross reactivity against a-1-anti-Trypsin from other sources is unknown.
P01009 - UniProtKB
P01009.3 - NCBI Protein
5265 - Gene ID

Application Details

ELISA, WB
Anti-Alpha-1-Anti-Trypsin has been tested by ELISA and western blot. This product is assayed against 1.0 ug of a-1-anti-Trypsin in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:8,000 to 1:29,000 of the reconstitution concentration is suggested for this product.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
A member of the serpin family, Anti-Alpha-1-Anti-Trypsin inhibits serine proteases. With a moderate affinity for plasmin and thrombin, Anti-Alpha-1-Anti-Trypsin antibody mainly targets elastase. It inhibits trypsin, chymotrypsin and plasminogen activator by inhibiting insulin-induced NO synthesis. It also decreases coagulation time and has proteolytic activity against insulin and plasmin. The short peptide from AAT also targets elastase, but not trypsin. Anti-Alpha-1-Anti-Trypsin is ideal for researchers involved in Metabolism, Cancer, and Cardiovascular research.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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