AHA1 Antibody
Anti-AHA1 Antibody - Western Blot
Rabbit anti-AHA1 WB
Western Blot of Anti-AHA1
Western Blot of Anti-AHA1
Western Blot of Anti-AHA1
Western Blot of Anti-AHA1
Western Blot of Anti-AHA1
Western Blot of Anti-AHA1
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Datasheet

AHA1 Antibody

Rabbit Polyclonal

600-401-974
100 µg
ELISA, WB, IHC, IP
Human, Monkey
Rabbit
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$399.00 /Per Item
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo.  The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone.  Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle.  Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370–390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone.  Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER).  Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 38-40 kDa in size corresponding to AHA1 protein by western blotting in the appropriate cell lysate or extract.

Purity/Specificity

This affinity purified antibody is directed against human AHA1 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross-reactivity with AHA1 protein from human and chimpanzee based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.

Anti-AHA1 (RABBIT) Antibody - 600-401-974
rabbit anti-AHA1 Antibody, Aha-1, Aha 1, Ahsa1 antibody, Activator of Hsp90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
AHSA1
ELISA, WB
IHC, IP
Human
Human, Monkey
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human AHA1 protein.
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Xu W et al. Hsp90 middle domain phosphorylation initiates a complex conformational program to recruit the ATPase-stimulating cochaperone Aha1. Nat Commun. (2019)
Applications
WB, IB, PCA
Bachman et al. Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation. Nature Communications (2018)
Applications
IP, Co-IP
Woodford et al. The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding. Nature Communications (2016)
Applications
IHC, ICC, Histology
Prince et al. Client Proteins and Small Molecule Inhibitors Display Distinct Binding Preferences for Constitutive and Stress-Induced HSP90 Isoforms and Their Conformationally Restricted Mutants. PLOS One (2015)
Applications
IP, Co-IP; WB, IB, PCA

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If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.