ABCB5 Antibody
IHC - ABCB5 Antibody
Western Blot - ABCB5 Antibody
Immunohistochemistry - anti ABCB5 antibody
Immunohistochemistry - anti ABCB5 antibody
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Datasheet

ABCB5 Antibody

Rabbit Polyclonal

600-401-A77S 600-401-A77
25 µL 100 µg
ELISA, IHC, WB
Human
Rabbit
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$99.00 /Per Item
$399.00 /Per Item
25 µL $99.00
100 µg $399.00
Availability: Ships next business day Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-ABCB5 Antibody is a ATP-Binding Cassette protein. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5. ABCB5 antibody is ideal for Cancer and Signal Transduction research.

Application Note

This affinity purified ABCB5 antibody has been tested for use in ELISA, IHC, and western blotting.  Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human ABCB5 protein.  A BLAST analysis was used to suggest partial cross-reactivity with ABCB5 from monkey (85% homology), rat (68% homology) and mouse (62% homology) sources.  Cross-reactivity with ABCB5 from other sources has not been determined.

Anti-ABCB5 (RABBIT) Antibody - 600-401-A77
rabbit anti-ABCB5 Antibody, ABCB 5, ABCB-5, ATP binding cassette sub family B (MDR/TAP) member 5 antibody, ATP binding cassette sub family B member 5 antibody, EST422562 antibody, P glycoprotein ABCB5 antibody
ABCB5
ELISA, IHC, WB
Human
Human
Rabbit
Polyclonal
IgG
Peptide
ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.
Liquid (sterile filtered)
1.1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store Anti-ABCB5 at -20° C prior to opening. Aliquot antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Fukunaga-Kalabis M et al. Tenascin-C promotes melanoma progression by maintaining the ABCB5-positive side population. Oncogene. (2010)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.