TrueBlot® Anti-Rabbit Ig IP Agarose Bead
Western Blot of TrueBlot® Anti-Rabbit Ig IP Agarose Beads.
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Datasheet

TrueBlot® Anti-Rabbit Ig IP Agarose Bead

Goat Polyclonal

00-8800-25
2.5 mL
IP, SDS-PAGE, WB, ChIP
Rabbit
Goat
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$197.00 /Per Item
Availability: Ships next business day
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

TrueBlot® Anti-Rabbit Ig IP Agarose Beads are a suspension of activated agarose beads coupled with goat Anti-rabbit IgG. It is suitable for precipitation of rabbit IgGs used as the primary antibodies in immunoprecipitation assays. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

Application Note

Upon initial use of this product, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. Procedure: Preparation of Immunoprecipitated Sample for SDS-PAGE: 1. Preclear cell lysate: Add 50 μL of Anti-Rabbit IgG Beads and 500μl of cell lysate sample to a microcentrifuge tube and incubate on ice for 30 minutes. Spin at 10,000xg for 3 minutes and transfer the supernatant to a new microcentrifuge tube. 2. Immunoprecipitation: Add 5 μg of primary antibody to the microcentrifuge tube containing the precleared lysate. Incubate on ice for 1 hour. Add 50 μL of Anti-Rabbit IgG Beads. Incubate for 1 hour on a rocking platform. Spin the microcentrifuge tube at 10,000xg for 1 minute. Remove supernatant completely and wash the (pelleted) beads 3 times with 500 μL of Lysis Buffer (50mM Tris HCl, pH 8.0; 150mM NaCl; 1% NP-40). 3. Prepare sample for SDS-PAGE: After the last wash, aspirate supernatant, and add 50 μL Laemmli Buffer (with 50 mM DTT or 2% β-mercaptoethanol, final) to bead pellet. Vortex and heat to 90-100 °C for 10 minutes. Spin at 10,000xg for 3 minutes, collect supernatant, and load onto the gel. Avoid loading Anti-Rabbit Ig Beads. Note: The supernatant can be stored at -20 °C for future use. After thawing, add fresh dithiothreitol and heat as above. Centrifuge the sample at 10,000xg for 1 minute in a microcentrifuge tube to pellet any Anti-Rabbit Ig Beads and immediately transfer an aliquot of the supernatant to gel wells.

Purity/Specificity

TrueBlot® Anti-Rabbit Ig IP Agarose Beads have been tested in SDS-Page, immunoprecipitation, and western blot.

TrueBlot® Anti-Rabbit Ig IP Agarose Beads - 00-8800-25
Anti-Rabbit Immunoglobulin Gamma, Agarose-conjugated IgG, Gt-a-Rb IgG, Goat-anti-Rabbit IgG, TrueBlot for immunoprecipitation, IP beads for TrueBlot, Anti-Rabbit IgG IP agarose
IP, SDS-PAGE, WB
ChIP
Rabbit
Rabbit
Goat
Agarose ULTRA
Polyclonal
IgG
Immunoprecipitation Kit
Suspension of agarose beads
8.9mg antibody per cc agarose
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.09% (w/v) Sodium Azide
None
Wet Ice
Store vial at 4 °C prior to opening. DO NOT FREEZE.
Expiration date is six (6) months from date of opening.
Zheng X. et al. Upregulation of OATP1A2 in human oesophageal squamous cell carcinoma cells via the HDAC6-GCN5/PCAF-H3K9Ac axis. Xenobiotica. (2021)
Applications
IP, Co-IP
Nassal DM et al. Ca2+/calmodulin kinase II–dependent regulation of βIV-spectrin modulates cardiac fibroblast gene expression, proliferation, and contractility. J Biol Chem. (2021)
Applications
IP, Co-IP
Rodriguez GY et al. PFTK1 kinase regulates axogenesis during development via RhoA activation. bioRxiv Preprint (2021)
Applications
IP, Co-IP; Electronic Absorption and Fluorescence Spectroscopy
Alvandi Z., Opas M. c-Src kinase inhibits osteogenic differentiation via enhancing STAT1 stability. PLoS One (2020)
Applications
IP, Co-IP
Chu CS et al. Unique Immune Cell Coactivators Specify Locus Control Region Function and Cell Stage. Mol Cell (2020)
Applications
IP, Co-IP
Zheng JP et al. YY1 directly interacts with myocardin to repress the triad myocardin/SRF/CArG box-mediated smooth muscle gene transcription during smooth muscle phenotypic modulation. Sci Rep. (2020)
Applications
IP, Co-IP
Yi SA. et al. Fermented ginseng extract, BST204, disturbs adipogenesis of mesenchymal stem cells through inhibition of S6 kinase 1 signaling. J Ginseng Res. (2020)
Applications
IP, Co-IP; WB, IB, PCA
Akiyama H. et al. Control of cell migration by the novel protein phosphatase-2A interacting protein inka2. Cell Tissue Res. (2020)
Applications
IP, Co-IP
Weiterer SS, Meier-Soelch J, Georgomanolis T, et al. Distinct IL-1α-responsive enhancers promote acute and coordinated changes in chromatin topology in a hierarchical manner. EMBO J. (2020)
Applications
IP, Co-IP
Amano H et al. Telomere Dysfunction Induces Sirtuin Repression that Drives Telomere-Dependent Disease. Cell Metab. (2019)
Applications
IP, Co-IP
Zhu Q et al. Regulation of OCT2 transcriptional repression by histone acetylation in renal cell carcinoma. Epigenetics (2019)
Applications
ChIP
Chern YJ et al. The interaction between SPARC and GRP78 interferes with ER stress signaling and potentiates apoptosis via PERK/eIF2α and IRE1α/XBP-1 in colorectal cancer. Nature (2019)
Applications
IP, Co-IP
Mayr-Buro C et al. Single-Cell Analysis of Multiple Steps of Dynamic NF-κB Regulation in Interleukin-1α-Triggered Tumor Cells Using Proximity Ligation Assays. Cancers (Basel) (2019)
Applications
IP, Co-IP
Rueda EM, Hall BM, Hill MC, et al. The Hippo Pathway Blocks Mammalian Retinal Müller Glial Cell Reprogramming. Cell Rep. (2019)
Applications
IP, Co-IP
Sciuto MR et al. Two-Step Coimmunoprecipitation (TIP) Enables Efficient and Highly Selective Isolation of Native Protein Complexes. Mol Cell Proteomics (2018)
Applications
IP, Co-IP
Logan C et al. Functional role for stable microtubules in lens fiber cell elongation. Exp Cell Res (2018)
Applications
IP, Co-IP
Scheri K et al. c-MET receptor as potential biomarker and target molecule for malignant testicular germ cell tumors. Oncotarget (2018)
Applications
IP, Co-IP
Moriya et al. PRDM14 directly interacts with heat shock proteins HSP90α and glucose-regulated protein 78. Cancer Science (2018)
Applications
IP, Co-IP; WB, IB, PCA
Cervin et al. GM1 ganglioside-independent intoxication by Cholera toxin. PLOS Pathogens (2018)
Applications
IP, Co-IP
Peña-Philippides et al. Inhibition of MicroRNA-155 Supports Endothelial Tight Junction Integrity Following Oxygen-Glucose Deprivation. Journal of the American Heart Association (2018)
Applications
IP, Co-IP
Tykesson E, Hassinen A, Zielinska K, et al. Dermatan sulfate epimerase 1 and dermatan 4-O-sulfotransferase 1 form complexes that generate long epimerized 4-O-sulfated blocks. J Biol Chem. (2018)
Applications
IP, Co-IP
Zhai et al. NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene (2017)
Applications
IP, Co-IP
Emmett et al. Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge. Nature (2017)
Applications
IP, Co-IP
Armour et al. An HDAC3-PROX1 corepressor module acts on HNF4α to control hepatic triglycerides. Nature Communications (2017)
Applications
IP, Co-IP; WB, IB, PCA
Lee et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature (2016)
Applications
IP, Co-IP
Wang et al. Skin Keratins. Methods in Enzymology (2016)
Applications
IP, Co-IP
Whitt et al. BK channel inactivation gates daytime excitability in the circadian clock. Nature Communications (2016)
Applications
IP, Co-IP
Hwang et al. Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes. Journal of Biological Chemistry (2016)
Applications
IP, Co-IP; WB, IB, PCA
Wands AM et al. Fucosylation and protein glycosylation create functional receptors for cholera toxin. Elife (2015)
Applications
IP, Co-IP
Jabir et al. Mitochondrial damage contributes to Pseudomonas aeruginosa activation of the inflammasome and is downregulated by autophagy. Autophagy (2015)
Applications
IP, Co-IP
Sugawara S, Ito T, Sato S, et al. Generation of aminoterminally truncated, stable types of bioactive bovine and porcine fibroblast growth factor 4 in Escherichia coli. Biotechnol Appl Biochem. (2015)
Applications
IP, Co-IP
Basu S et al. Suppression of MAPK/JNK-MTORC1 signaling leads to premature loss of organelles and nuclei by autophagy during terminal differentiation of lens fiber cells. Autophagy (2014)
Applications
IP, Co-IP
Nicholas C et al. PRMT5 is upregulated in malignant and metastatic melanoma and regulates expression of MITF and p27(Kip1.) PLoS One (2013)
Applications
IP, Co-IP
Gao Y, Koppen A, Rakhshandehroo M, et al. Early adipogenesis is regulated through USP7-mediated deubiquitination of the histone acetyltransferase TIP60. Nat Commun. (2013)
Applications
IP, Co-IP
Rzeczkowski K, Beuerlein K, Müller H, et al. c-Jun N-terminal kinase phosphorylates DCP1a to control formation of P bodies. J Cell Biol. (2011)
Applications
IP, Co-IP
Shao J et al. Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation. Mol Cell Biol (2008)
Applications
IP, Co-IP
Wolter S, Doerrie A, Weber A, et al. c-Jun controls histone modifications, NF-kappaB recruitment, and RNA polymerase II function to activate the ccl2 gene. Mol Cell Biol. 2008;28(13):4407-4423. doi:10.1128/MCB.00535-07 Mol Cell Biol. (2008)
Applications
IP, Co-IP
Nelson RF, Glenn KA, Zhang Y, et al. Selective cochlear degeneration in mice lacking the F-box protein, Fbx2, a glycoprotein-specific ubiquitin ligase subunit. J Neurosci. (2007)
Applications
IP, Co-IP

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.