TrueBlot® Anti-Mouse Ig IP Agarose Beads
Immunoprecipitation/Western Blot of Anti-Mouse IgG IP Agarose Beads
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Datasheet

TrueBlot® Anti-Mouse Ig IP Agarose Beads

Goat Polyclonal

00-8811-25
2.5 mL
IP, SDS-PAGE, WB, Biochemical Assay
Mouse
Goat
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$197.00 /Per Item
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

TrueBlot® Anti-Mouse Ig IP Agarose Beads are a suspension of activated agarose beads coupled with goat Anti-mouse IgG. It is suitable for precipitation of mouse IgGs used as the primary antibodies in immunoprecipitation assays. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

Application Note

Upon initial use of this product, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. Procedure: Preparation of Immunoprecipitated Sample for SDS-PAGE: 1. Preclear cell lysate: Add 50 μL of Anti-Mouse IgG Beads and 500 μL of cell lysate sample to a microcentrifuge tube and incubate on ice for 30 minutes. Spin at 10,000xg for 3 minutes and transfer the supernatant to a new microcentrifuge tube. 2. Immunoprecipitation: Add 5 μg of primary antibody to the microcentrifuge tube containing the precleared lysate. Incubate on ice for 1 hour. Add 50 μL of Anti-Mouse IgG Beads. Incubate for 1 hour on a rocking platform. Spin the microcentrifuge tube at 10,000xg for 1 minute. Remove supernatant completely and wash the (pelleted) beads 3 times with 500 μL of Lysis Buffer (50mM Tris HCl, pH 8.0; 150mM NaCl; 1% NP-40). 3. Prepare sample for SDS-PAGE: After the last wash, aspirate supernatant, and add 50 μL Laemmli Buffer (with 50 mM DTT or 2% β-mercaptoethanol, final) to bead pellet. Vortex and heat to 90-100 °C for 10 minutes. Spin at 10,000xg for 3 minutes, collect supernatant, and load onto the gel. Avoid loading Anti-Mouse Ig Beads. Note: The supernatant can be stored at -20 °C for future use. After thawing, add fresh dithiothreitol and heat as above. Centrifuge the sample at 10,000xg for 1 minute in a microcentrifuge tube to pellet any Anti-Mouse Ig Beads and immediately transfer an aliquot of the supernatant to gel wells.

Purity/Specificity

TrueBlot® Anti-Mouse Ig IP Agarose Beads has been tested in SDS-Page, immunoprecipitation, and western blot.

TrueBlot® Anti-Mouse Ig IP Agarose Beads - 00-8811-25
Anti-Mouse immunoglobulin Gamma, Agarose-conjugated IgG, Gt-a-Ms IgG, Goat anti-Mouse IgG, TrueBlot, TrueBlot for immunoprecipitation, IP Agarose beads for TrueBlot.
IP, SDS-PAGE, WB
Biochemical Assay
Mouse
Mouse
Goat
Agarose ULTRA
Polyclonal
IgG
Immunoprecipitation Kit
Suspension of agarose beads
14.4 mg/cc antibody per cc agarose
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.09% (w/v) Sodium Azide
None
Wet Ice
Store vial at 4 °C prior to opening. DO NOT FREEZE.
Expiration date is six (6) months from date of opening.
Rodriguez GY et al. PFTK1 kinase regulates axogenesis during development via RhoA activation. bioRxiv Preprint (2021)
Applications
IP, Co-IP
Nin DS et al. GAGE mediates radio resistance in cervical cancers via the regulation of chromatin accessibility. Cell Rep. (2021)
Applications
IP, Co-IP
Yao F et al. A targetable LIFR− NF-κB− LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis. Nat Commun. (2021)
Applications
IP, Co-IP
Akiyama H. et al. Control of cell migration by the novel protein phosphatase-2A interacting protein inka2. Cell Tissue Res. (2020)
Applications
IP, Co-IP
Huo YN et al. Androgen receptor activation reduces the endothelial cell proliferation through activating the cSrc/AKT/p38/ERK/NFκB-mediated pathway. J Steroid Biochem Mol Biol (2019)
Applications
IP, Co-IP
Zhai et al. NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene (2017)
Applications
IP, Co-IP
Hancock et al. Co-regulation of mitochondrial respiration by proline dehydrogenase/oxidase and succinate. Amino Acids (2016)
Applications
IP, Co-IP
Zhao et al. MLL5 maintains spindle bipolarity by preventing aberrant cytosolic aggregation of PLK1. Journal of Cell Biology (2016)
Applications
IP, Co-IP; WB, IB, PCA
Manne et al. Distinct pathways regulate Syk protein activation downstream of immune tyrosine activation motif (ITAM) and hemITAM receptors in platelets. Journal of Biological Chemistry (2015)
Applications
Kinase Assay
Ziesche E et al. The coactivator role of histone deacetylase 3 in IL-1-signaling involves deacetylation of p65 NF-κB. Nucleic Acids Res (2013)
Applications
IP, Co-IP
Wolf A et al. Identification and Functional Characterization of Novel Phosphorylation Sites in TAK1-Binding Protein (TAB) 1. PLoS One (2011)
Applications
IP, Co-IP
Rzeczkowski K, Beuerlein K, Müller H, et al. c-Jun N-terminal kinase phosphorylates DCP1a to control formation of P bodies. J Cell Biol. (2011)
Applications
IP, Co-IP

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.