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Nitrotyrosine ELISA Kit

KOA0888
1 Kit
ELISA
Broad
62.5 ng/ml - 8000 nM
$609.00 /Per Item
Out of stock
Ships in approximately 14 days
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Nitrotyrosine AccuSignal ELISA Kit - KOA0888
Nitro tyrosine ELISA Kit, Nitrosylation, NOX, Nitrotyrosine ELISA KIT, Nitrotyrosine Enzyme-Linked Immunosorbent Assay, 3-Nitrotyrosine ELISA Kit, 3-Nitro-L-tyrosine ELISA Kit, 3 Nitrotyrosine ELISA Kit, 3-NT ELISA Kit, 3NT ELISA Kit
Peroxidase (HRP)
Monoclonal
ELISA Kit
62.5 ng/ml - 8000 nM

Target Details

Broad
This kit contains: 96 wells coated plate, Target Protein Standard, HRP Conjugated Monoclonal Antibody, Diluent, Wash buffer, TMB Substrate and stop reagent, see protocol for additional information.

Application Details

ELISA
Colorimetric Competitive ELISA (Enzyme-linked Immunosorbent Assay) assay for quantification of Free Nitrotyrosine in Cell lysates, Plasma, Serum, and Urine. Sensitivity: 50nM. Range: 62.5-8000nM.

Shipping & Handling

Wet Ice
See kit insert for component storage. Store kit at 4°C and -20°C.
Expiration date is one (1) year from date of receipt.
Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.

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