NFkB Antibody Sampler Kit
NFKB Antibody Sampler Kit-Kits and Buffers
NFKB Antibody Sampler Kit-Kits and Buffers
NFKB Antibody Sampler Kit-Kits and Bufferst
NFKB Antibody Sampler Kit-Kits and Buffers- Western Blot
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Datasheet

NFkB Antibody Sampler Kit

K711
1 Kit
WB
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$912.00 /Per Item
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells. IKB-alpha inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, IKBA becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. Anti-NFkB and Anti-IKBa Antibodies are ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction and Immunology research.

Application Note

Each kit contains one unit of the following products: Anti-NFKB p50 (NFKB1) (RABBIT) Antibody (100 µL) Anti-NFKB p65 (Rel A) (RABBIT) Antibody (100 µL) Anti-NFKB cRel (RABBIT) Antibody (100 µL) Anti-IKBα C-terminal (RABBIT) Antibody (100 µL) Anti-RABBIT IgG (H&L) (GOAT) Peroxidase Conjugated Antibody (100 µg) CONTROL PEPTIDE for Anti-NFKB p50 (NFKB1) (RABBIT) Antibody (50 µg) CONTROL PEPTIDE for Anti-NFKB p65 (Rel A) (RABBIT) Antibody (50 µg) CONTROL PEPTIDE for Anti-NFKB cRel (RABBIT) Antibody (50 µg) CONTROL PEPTIDE for Anti-IKBα C-terminal (RABBIT) Antibody ( (50 µg)

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

NFKB ANTIBODY SAMPLER KIT - K711
Anti-NFKB p50, Anti-NFKB p65, Rabbit Anti-NFKB cRel, Anti-IKBalpha, Control peptides, antibody sample kits
WB
Liquid (sterile filtered)
Dry Ice
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.