Mouse TrueBlot® Set (IP Agarose beads)
Mouse TrueBlot® IP / Western Blot
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Datasheet

Mouse TrueBlot® Set (IP Agarose beads)

Monoclonal eB144

88-7788-31
1 Set
IP, WB
Mouse
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$293.00 /Per Item
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Description

Background

Mouse IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Mouse IgG monoclonal secondary antibody. Mouse IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Mouse IgG TrueBlot®, simply substitute the conventional HRP Anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Mouse IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Mouse IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.

Application Note

Mouse IgG TrueBlot® ULTRA is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. Mouse TrueBlot Set Components: 1. Mouse IgG TrueBlot® HRP-conjugated monoclonal secondary antibody reacting with mouse IgGs for optimal signal detection in immunoprecipitation/immunoblotting experiments. 2. Anti-Mouse Ig IP Beads: 2.5 mL. Binds 0.4 mg Ig/mL beads. 3. Western blot incubation tray. Special Notes: Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution factor for your desired application. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.

Purity/Specificity

Mouse TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA.

Storage Condition

Store TrueBlot® Anti-Mouse Ig IP beads (00-8811-25) at 2-8 °C and Mouse TrueBlot® (18-8817-31) at -20 °C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.

Mouse TrueBlot® Set (with IP Agarose beads) - 88-7788-31
Anti-Mouse immunoglobulin Gamma, Agarose-conjugated IgG, Anti-Ms IgG, Anti-Mouse IgG HRP, TrueBlot, TrueBlot for immunoprecipitation, IP Agarose beads for TrueBlot, HRP, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for western blotting, Anti-Mouse IgG IP Agarose
IP, WB
Mouse
Mouse
Peroxidase (Horseradish) ULTRA
Monoclonal
eB144
IgG
Immunoprecipitation Kit
Liquid (sterile filtered)
Wet Ice
Expiration date is six (6) months from date of opening.
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Kiran S et al. The deubiquitinase USP46 is essential for proliferation and tumor growth of HPV-transformed cancers. (2018)
Applications
IP, Co-IP
Granados-Durán et al. Complement system activation contributes to the ependymal damage induced by microbial neuraminidase. (2016)
Applications
IP, Co-IP
Braun A et al. High-resolution time-lapse imaging and automated analysis of microtubule dynamics in living human umbilical vein endothelial cells. (2016)
Applications
IP, Co-IP

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.